Effects of exogenous donor of nitric oxide-sodium nitroprusside on energy production of rat reticulocytes.

Effects of exogenous donor of nitric oxide-sodium nitroprusside on energy production of rat reticulocytes. Research Abstract Details 

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Effects of exogenous donor of nitric oxide-sodium nitroprusside on energy production of rat reticulocytes. Abstract Text:

S D Maleti?,L M Dragi?evi?-Djokovi?,B L Ognjanovi?,R V Ziki?,A S Stajn,M B Spasi?,

The effects of the sodium nitroprusside (SNP), a nitric oxide (NO) donor clinically used in the treatment of hypertensive emergencies on the energy production of rat reticulocytes were investigated. Rat reticulocyte-rich red blood cell suspensions were aerobically incubated without (control) or in the presence of different concentrations of SNP (0.1, 0.25, 0.5, 1.0 mM). SNP decreased total and coupled, but increased uncoupled oxygen consumption. This was accompanied by the stimulation of glycolysis, as measured by increased glucose consumption and lactate accumulation. Levels of all glycolytic intermediates indicate stimulation of hexokinase-phosphofructo kinase (HK-PFK), glyceraldehyde 3-phosphate dehydrogenase (GAPD) and pyruvate kinase (PK) activities in the presence of SNP. Due to the decrease of coupled oxygen consumption in the presence of SNP, ATP production via oxidative phosphorylation was significantly diminished. Simultaneous increase of glycolytic ATP production was not enough to provide constant ATP production. In addition, SNP significantly decreased ATP level, which was accompanied with increased ADP and AMP levels. However, the level of total adenine nucleotides was significantly lower, which was the consequence of increased catabolism of adenine nucleotides (increased hypoxanthine level). ATP/ADP ratio and adenylate energy charge level were significantly decreased. In conclusion, SNP induced inhibition of oxidative phosphorylation, stimulation of glycolysis, but depletion of total energy production in rat reticulocytes. These alterations were accompanied with instability of energy status.

Effects of exogenous donor of nitric oxide-sodium nitroprusside on energy production of rat reticulocytes. Publishing Authors By Initials

SD Maleti?,LM Dragi?evi?-Djokovi?,BL Ognjanovi?,RV Ziki?,AS Stajn,MB Spasi?,

For similar cells: blood cells: erythrocytes: reticulocytes research abstracts see: cells: blood cells: erythrocytes: reticulocytes research

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Effects of exogenous donor of nitric oxide-sodium nitroprusside on energy production of rat reticulocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Physiological research / Academia Scientiarum Bohe

VOLUME: 53

Page Numbers: 439-47

Journal Abbreviation: Physiol Res

ISSN: 0862-8408

DAY: 2

MONTH: 04

YEAR: 2004

Effects of exogenous donor of nitric oxide-sodium nitroprusside on energy production of rat reticulocytes. Information

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LANGUAGE: eng

NlmUniqueID: 9112413

Effects of exogenous donor of nitric oxide-sodium nitroprusside on energy production of rat reticulocytes. Keywords Mesh Terms:

KEYWORDS: Reticulocytes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Effects of exogenous donor of nitric oxide-sodium nitroprusside on energy production of rat reticulocytes. Information

Substance Name: Nitroprusside

Registry Number: 15078-28-1

Grant and Affiliation Information for Effects of exogenous donor of nitric oxide-sodium nitroprusside on energy production of rat reticulocytes.

AFFILIATION: Institute of Biology, Faculty of Sciences, Radoje Domanovi? 12, 34000 Kragujevac, P.O.Box 60, Serbia and Montenegro. maletic@knez.uis.kg.ac.yu

Country: Czech Republic

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MEDLINETA: Physiol Res

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