Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin.

Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin. Abstract Text:

K Tsurugi,K Ogata,

A neutral protease, named protease B in the previous report (Tsurugi, K. & Ogata, K. (1982) J. Biochem. 92, 1369-1381), was partially purified from rat liver chromatin by gel filtration through Sepharose 6B followed by DE-Sephadex column chromatography. The proteolytic activity on total histones of the partially-purified protease B was increased about two fold by addition of DNA and again increased by further addition of 2 M urea. Analysis of the hydrolysed products showed that out of five species of histones, only H1 was degraded in the presence of an amount of DNA equivalent to the amount of histones, whereas core histones were also degraded in the absence or presence of one-tenth amount of DNA. Urea accelerated the selective degradation of H1 histone because H1 histone was preferentially degraded in the presence of even a low amount of DNA. In contrast, core histones became resistant to the protease B in the presence of DNA and/or urea. Heat-denatured DNA stimulated the degradation of H1 histone even in the absence of urea to almost the same extent that native DNA did in the presence of urea. Thus, protease B efficiently degrades H1 histone when its association with DNA is destabilized by either addition of urea or pretreatment of DNA with heat.

Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin. Publishing Authors By Initials

K Tsurugi,K Ogata,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

PUBMED ID PMID:

MEDLINE DATE:

Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 99

Page Numbers: 237-41

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1986

Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin. Information

Substance Name: histone proteases

Registry Number: EC 3.4.99.-

Grant and Affiliation Information for Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Effects of DNA and urea on the specificity for H1 histone of the neutral protease B partially-purified from rat liver chromatin Related Publications

• Androgen receptor stimulates bone sialoprotein (BSP) gene transcription via cAMP response element and activator protein 1/glucocorticoid response elements.
• Biological process of carbohydrate attachment to ovalbumin.
• Complete cDNA sequence of the fourth component of murine complement.
• Constitutive expression of Slp genes in mouse strain B10.WR directed by C4 regulatory sequences.
• Effects of essential medicines on cardiovascular products available for the market in Thailand.
• Effects of protein deficiency on serum albumin-synthesizing activity of rat liver--with special reference to the presence of transcriptional controls.
• Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver.
• Effects of the use of high concentrations of Mg2+ in the preparation of proteins from rat liver ribosomes pretreated with polyvinyl sulfate. Improved methods for preparing ribosomal proteins from ribosomal precursor particles of liver nucleoli.
• Evaluation of cleft lip bony depression of piriform margin and nasal deformity with cone beam computed tomography: "retruded-like" appearance and anteroposterior position of the alar base.
• Evidence for exclusive biosynthesis in vivo of serum albumin by bound polysomes of rat liver.
• Fermi-surface reconstruction without breakdown of Kondo screening at the quantum critical point.
• Hemoglobin biosynthesis in the chicken red blood cells and inhibitory effect of actinomycin D.
• Incorporation of 14C-amino acids into basic proteins of 47S and 32S subunits of rat liver ribosomes by a cell-free system.
• Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo).
• Lack of exchange of ribosomal structural proteins with cell-sap proteins of rat liver in vitro.
• Malignant melanoma in a woman with LEOPARD syndrome: identification of a germline PTPN11 mutation and a somatic BRAF mutation.
• Mice constitutive for sex-limited protein (SLP) expression contain multiple Slp gene sequences.
• Preferential synthesis of arginase by free polysomes from rat liver.
• Purification and properties of acylamino acid-releasing enzyme from rat liver.
• Rat liver nucleolar 29.5S RNA as the precursor of nucleolar 28S RNA.
• Stabilizing effect of ribonuclease inhibitor on structure of polysomes and some properties of four classes of ribosomal particles in rat liver cytoplasm.
• Structure and expression of murine fourth complement component (C4) and sex-limited protein (Slp).
• Studies on SDS-phenol methods for extraction of rat liver nuclear RNA. I. Purity, recovery, and specific radioactivity of pulse labeled nuclear RNA obtained by SDS-phenol extraction under various conditions.
• Studies on SDS-phenol methods for extraction of rat liver nuclear RNA. II. Polyacrylamide gel electrophoresis of nuclear RNA obtained using various conditions for SDS-phenol extraction.
• Studies on creatine kinase of skeletal muscle and brain with special reference to subcellular distribution and isozymes.
• Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver.
• The different effects of N-ethylmaleimide and iodoacetamide on the activity of rat liver 60S subunits for peptide bond elongation.
• Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells.
• [Sudden cardiac death]
• cDNA sequence of a novel sex-limited protein (Slp) from mice constitutive for Slp expression. Sequence comparisons suggest that Slp has no functional role.