Effects of Disruption of Homocitrate Synthase Genes on Nostoc sp. Strain PCC 7120 Photobiological Hydrogen Production and Nitrogenase.

Effects of Disruption of Homocitrate Synthase Genes on Nostoc sp. Strain PCC 7120 Photobiological Hydrogen Production and Nitrogenase. Research Abstract Details 

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Effects of Disruption of Homocitrate Synthase Genes on Nostoc sp. Strain PCC 7120 Photobiological Hydrogen Production and Nitrogenase. Abstract Text:

Hajime Masukawa,Kazuhito Inoue,Hidehiro Sakurai,Hajime Masukawa,Kazuhito Inoue,Hidehiro Sakurai,Hajime Masukawa,Kazuhito Inoue,Hidehiro Sakurai,

In the case of nitrogenase-based photobiological hydrogen production systems of cyanobacteria, the inactivation of uptake hydrogenase (Hup) leads to significant increases in hydrogen production activity. However, the high-level-activity stage of the Hup mutants lasts only a few tens of hours under air, a circumstance which seems to be caused by sufficient amounts of combined nitrogen supplied by active nitrogenase. The catalytic FeMo cofactor of nitrogenase binds homocitrate, which is required for efficient nitrogen fixation. It was reported previously that the nitrogenase from the homocitrate synthase gene (nifV) disruption mutant of Klebsiella pneumoniae shows decreased nitrogen fixation activity and increased hydrogen production activity under N(2). The cyanobacterium Nostoc sp. strain PCC 7120 has two homocitrate synthase genes, nifV1 and nifV2, and with the DeltahupL variant of Nostoc sp. strain PCC 7120 as the parental strain, we have constructed two single mutants, the DeltahupL DeltanifV1 strain (with the hupL and nifV1 genes disrupted) and the DeltahupL DeltanifV2 strain, and a double mutant, the DeltahupL DeltanifV1 DeltanifV2 strain. Diazotrophic growth rates of the two nifV single mutants and the double mutant were decreased moderately and severely, respectively, compared with the rates of the parent DeltahupL strain. The hydrogen production activity of the DeltahupL DeltanifV1 mutant was sustained at higher levels than the activity of the parent DeltahupL strain after about 2 days of combined-nitrogen step down, and the activity in the culture of the former became higher than that in the culture of the latter. The presence of N(2) gas inhibited hydrogen production in the DeltahupL DeltanifV1 DeltanifV2 mutant less strongly than in the parent DeltahupL strain and the DeltahupL DeltanifV1 and DeltahupL DeltanifV2 mutants. The alteration of homocitrate synthase activity can be a useful strategy for improving sustained photobiological hydrogen production in cyanobacteria.

Effects of Disruption of Homocitrate Synthase Genes on Nostoc sp. Strain PCC 7120 Photobiological Hydrogen Production and Nitrogenase. Publishing Authors By Initials

H Masukawa,K Inoue,H Sakurai,H Masukawa,K Inoue,H Sakurai,H Masukawa,K Inoue,H Sakurai,

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Effects of Disruption of Homocitrate Synthase Genes on Nostoc sp. Strain PCC 7120 Photobiological Hydrogen Production and Nitrogenase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Applied and environmental microbiology

VOLUME: 73

Page Numbers: 7562-70

Journal Abbreviation: Appl. Environ. Microbiol.

ISSN: 0099-2240

DAY: 12

MONTH: 10

YEAR: 2007

Effects of Disruption of Homocitrate Synthase Genes on Nostoc sp. Strain PCC 7120 Photobiological Hydrogen Production and Nitrogenase. Information

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LANGUAGE: eng

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AFFILIATION: Department of Biological Sciences, Kanagawa University, 2946 Tsuchiya, Hiratsuka, Kanagawa 259-1293, Japan. masukawa@kurenai.waseda.jp.

Country: United States

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MEDLINETA: Appl Environ Microbiol

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