Effects of cross-linking of membrane proteins on vesiculation induced by dimyristoylphosphatidylcholine in human erythrocytes.

Effects of cross-linking of membrane proteins on vesiculation induced by dimyristoylphosphatidylcholine in human erythrocytes. Research Abstract Details 

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Effects of cross-linking of membrane proteins on vesiculation induced by dimyristoylphosphatidylcholine in human erythrocytes. Abstract Text:

T Yamaguchi,S Yamada,E Kimoto,

To study the effect of cross-linking of membrane proteins on vesiculation of human erythrocytes by dimyristoylphosphatidylcholine (DMPC), red cells were treated with diamide at atmospheric pressure or 100 MPa and then incubated with DMPC in buffers of pH 6.5-8.5. Irrespective of buffer pH, the amount of released vesicles increased upon cross-linking of membrane proteins but approached the control level upon reduction of the cross-linking by dithiothreitol. Similar enhancement of vesicle release was also observed in N-ethylmaleimide-treated red cells. Hemolysis during vesiculation was observed only in red cells treated with diamide at 100 MPa. Furthermore, the composition of membrane proteins in released vesicles was analyzed by SDS-PAGE. Membrane vesicles released from intact red cells or the cells treated with diamide at atmospheric pressure contained band 3 as a major membrane protein. On the other hand, membrane vesicles from red cells treated with diamide at 100 MPa contained protein 4.1 in addition to band 3 and the orientation of these proteins was similar to that in intact cells. These results indicate that the amount and membrane protein composition of DMPC-induced vesicles are much affected by chemical modification of SH-groups in red cell membrane proteins.

Effects of cross-linking of membrane proteins on vesiculation induced by dimyristoylphosphatidylcholine in human erythrocytes. Publishing Authors By Initials

T Yamaguchi,S Yamada,E Kimoto,

For similar chemical actions and uses: specialty uses of chemicals: laboratory chemicals: indicators and reagents: sulfhydryl reagents research abstracts see: chemical actions and uses: specialty uses of chemicals: laboratory chemicals: indicators and reagents: sulfhydryl reagents research

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Effects of cross-linking of membrane proteins on vesiculation induced by dimyristoylphosphatidylcholine in human erythrocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 115

Page Numbers: 659-63

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1994

Effects of cross-linking of membrane proteins on vesiculation induced by dimyristoylphosphatidylcholine in human erythrocytes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Effects of cross-linking of membrane proteins on vesiculation induced by dimyristoylphosphatidylcholine in human erythrocytes. Keywords Mesh Terms:

KEYWORDS: Sulfhydryl Reagents

MESH TERMS: chemistry

Chemical & Substance for Abstract: Effects of cross-linking of membrane proteins on vesiculation induced by dimyristoylphosphatidylcholine in human erythrocytes. Information

Substance Name: 4,4'-Diisothiocyanostilbene-2,2'-Disulfo

Registry Number: 53005-05-3

Grant and Affiliation Information for Effects of cross-linking of membrane proteins on vesiculation induced by dimyristoylphosphatidylcholine in human erythrocytes.

AFFILIATION: Department of Chemistry, Faculty of Science, Fukuoka University.

Country: JAPAN

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MEDLINETA: J Biochem

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