Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells.

Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. Research Abstract Details 

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Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. Abstract Text:

Hidekazu Oshina,Shinichi Sotome,Toshitaka Yoshii,Ichiro Torigoe,Yumi Sugata,Hidetsugu Maehara,Eriko Marukawa,Ken Omura,Kenichi Shinomiya,

Human bone marrow-derived mesenchymal cells (hBMMCs) originate from cell populations in the bone marrow and are capable of differentiating along multiple mesenchymal lineages. To differentiate hBMMCs into osteoblasts, adipocytes and chondrocytes, dexamethasone has been used as a differentiation reagent. We hypothesized that dexamethasone would augment the responsiveness of BMMCs to other differentiation reagents and not define the lineage. This study investigated the effect of continuous treatment with 100 nM dexamethasone on the differentiation of BMMCs into three different lineages. hBMMCs cultured with continuous dexamethasone treatment (100 nM) exhibited higher mRNA expression levels of osteogenic markers and higher positive rates of colony forming unit assays for osteogenesis compared to hBMMCs treated with dexamethasone only during the differentiation culture. Furthermore, continuous dexamethasone treatment augmented bone formation capability of monkey-derived BMMCs in a bone induction experimental model at an extra skeletal site. In addition, continuously dexamethasone-treated hBMMCs formed larger chondrogenic pellets and expressed SOX9 at higher level than the control BMMCs. Likewise, continuous dexamethasone treatment facilitated adipogenic differentiation based on mRNA level and colony forming unit analysis. To investigate the mechanism of the augmentation of differentiation, further studies on apoptosis were conducted. The studies indicated that dexamethasone selectively induced apoptosis of some populations of hBMMCs which were thought to have poor differentiation capability.

Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. Publishing Authors By Initials

H Oshina,S Sotome,T Yoshii,I Torigoe,Y Sugata,H Maehara,E Marukawa,K Omura,K Shinomiya,

For similar physiological processes: growth and development: morphogenesis: embryonic and fetal development: organogenesis: musculoskeletal development: bone development: osteogenesis research abstracts see: physiological processes: growth and development: morphogenesis: embryonic and fetal development: organogenesis: musculoskeletal development: bone development: osteogenesis research

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Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Bone

VOLUME: 41

Page Numbers: 575-83

Journal Abbreviation: Bone

ISSN: 8756-3282

DAY: 10

MONTH: 07

YEAR: 2007

Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. Information

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LANGUAGE: eng

NlmUniqueID: 8504048

Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. Keywords Mesh Terms:

KEYWORDS: Osteogenesis

MESH TERMS: drug effects

Chemical & Substance for Abstract: Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells. Information

Substance Name: Caspase 3

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Effects of continuous dexamethasone treatment on differentiation capabilities of bone marrow-derived mesenchymal cells.

AFFILIATION: Oral and Maxillofacial Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.

Country: United States

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MEDLINETA: Bone

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