[Effects of compound Salvia injection on number and activity of endothelial progenitor cells]

[Effects of compound Salvia injection on number and activity of endothelial progenitor cells] Research Abstract Details 

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[Effects of compound Salvia injection on number and activity of endothelial progenitor cells] Abstract Text:

Kang-Ting Ji,Huai-Qin Zhang,Peng-Lin Yang,Hai-Ying Li,De-Ye Yang,Xiao-Yan Huang,

OBJECTIVE: To investigate the effects of Compound Salvia injection (CSI) on the number and activity of endothelial progenitor cells (EPCs). METHOD: Mononuclear fraction of human umbilical cord blood was obtained by density gradient centrifugation and plated on fibronectin coated culture dishes. Cells were divided in to five groups: group control, group VEGF, group CSI 50, group CSI 10 and group CSI 2 (supplemented with none cytokine, VEGF 10 ng x mL(-1), CSI 50, 10, 2 microg x mL(-1), respectively). After six days in culture, cell clusters were viewed with an inverted microscope, fluorescence-activated cell sorting (FACS) analysis of PE-CD34 and FITC-VE-Cadherin was performed to detect number of EPCs, adhesion assay was performed by replating cells on fibronectin coated dishes, and then counting adherent cells. RESULT: Numbers of EPCs of group VEGF, group CSI 10 and group CSI 2 were significantly increased as compared with those of group control ( P < 0.01, P < 0.05, P < 0.01, respectively), and numbers of EPCs of group CSI 2 were more than those of group CSI 10 and group V (P < 0.01). Compared with group control, number of EPCs of group CSI 50 was significantly decreased (P < 0.01). Compared with group control, numbers of clusters and adhesive EPCs of group CSI 10 and group CSI 2 were significantly increased, while those of group CSI 50 were significantly decreased. CONCLUSION: Low concentration CSI can significantly promote EPCs augmentation and enhance its functional activity, while high concentration CSI significantly restrains it.

[Effects of compound Salvia injection on number and activity of endothelial progenitor cells] Publishing Authors By Initials

KT Ji,HQ Zhang,PL Yang,HY Li,DY Yang,XY Huang,

For similar cells: stem cells research abstracts see: cells: stem cells research

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[Effects of compound Salvia injection on number and activity of endothelial progenitor cells] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazh

VOLUME: 31

Page Numbers: 246-9

Journal Abbreviation: Zhongguo Zhong Yao Za Zhi

ISSN: 1001-5302

DAY: 6

MONTH: Feb

YEAR: 2006

[Effects of compound Salvia injection on number and activity of endothelial progenitor cells] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 8913656

[Effects of compound Salvia injection on number and activity of endothelial progenitor cells] Keywords Mesh Terms:

KEYWORDS: Stem Cells

MESH TERMS: drug effects

Chemical & Substance for Abstract: [Effects of compound Salvia injection on number and activity of endothelial progenitor cells] Information

Substance Name: Drugs, Chinese Herbal

Registry Number: 0

Grant and Affiliation Information for [Effects of compound Salvia injection on number and activity of endothelial progenitor cells]

AFFILIATION: Department of Cardiology, the Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China. jktdtl@sohu.com

Country: China

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MEDLINETA: Zhongguo Zhong Yao Za Zhi

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