Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier.

Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier. Research Abstract Details 

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Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier. Abstract Text:

Luying Peng,Zhenjuan He,Wei Chen,Ian R Holzman,Jing Lin,

Production of short-chain fatty acids (SCFA) in the intestinal lumen may play an important role in the maintenance of the intestinal barrier. However, overproduction/accumulation of SCFA in the bowel may be toxic to the intestinal mucosa and has been hypothesized to play a role in the pathogenesis of neonatal necrotizing enterocolitis (NEC). By using a Caco-2 cell monolayer model of intestinal barrier, we report here that the effect of butyrate on the intestinal barrier is paradoxical. Butyrate at a low concentration (2 mM) promotes intestinal barrier function as measured by a significant increase in transepithelial electrical resistance (TER) and a significant decrease in inulin permeability. Butyrate at a high concentration (8 mM) reduces TER and increases inulin permeability significantly. Butyrate induces apoptosis and reduces the number of viable Caco-2 cells in a dose-dependent manner. Intestinal barrier function impairment induced by high concentrations of butyrate is most likely related to butyrate-induced cytotoxicity due to apoptosis. We conclude that the effect of butyrate on the intestinal barrier is paradoxical; i.e. whereas low concentrations of butyrate may be beneficial in promoting intestinal barrier function, excessive butyrate may induce severe intestinal epithelial cell apoptosis and disrupt intestinal barrier.

Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier. Publishing Authors By Initials

L Peng,Z He,W Chen,IR Holzman,J Lin,

For similar natural sciences: chemistry: chemistry, physical: permeability research abstracts see: natural sciences: chemistry: chemistry, physical: permeability research

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Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Pediatric research

VOLUME: 61

Page Numbers: 37-41

Journal Abbreviation: Pediatr. Res.

ISSN: 0031-3998

DAY: 3

MONTH: Jan

YEAR: 2007

Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100714

Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier. Keywords Mesh Terms:

KEYWORDS: Permeability

MESH TERMS: drug effects

Chemical & Substance for Abstract: Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier. Information

Substance Name: Inulin

Registry Number: 9005-80-5

Grant and Affiliation Information for Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier.

AFFILIATION: Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029, USA.

Country: United States

AGENCY: United States NICHD

GRANT: 1-R03-HD049881

ACRONYM: HD

MEDLINETA: Pediatr Res

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