Effects of bFGF incorporated into a gelatin sheet on wound healing.

Effects of bFGF incorporated into a gelatin sheet on wound healing. Research Abstract Details 

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Effects of bFGF incorporated into a gelatin sheet on wound healing. Abstract Text:

Michiyo Miyoshi,Takeshi Kawazoe,Hiroharu H Igawa,Yasuhiko Tabata,Yoshito Ikada,Shigehiko Suzuki,

Basic fibroblast growth factor (bFGF) is well known to promote the proliferation of almost all cells associated with wound healing. However, as the activation duration of bFGF is very short in vivo, we incorporated bFGF into an acidic gelatin hydrogel and studied the sustained release of bFGF in vivo. In addition, we investigated the effects of the acidic gelatin sheet containing bFGF on wound healing. To distinguish wound contraction from neoepithelialization, we measured both the wound area and neoepithelium length. Other histological parameters such as thickness of granulation tissue and number of capillaries were also determined as indices of wound healing. Fibrous tissue was assessed using an Elastica van Gieson and Azan stain. A skin defect (1.5 x 1.5 cm) of full thickness was created on the back of each test mouse and the wound was covered with an acidic gelatin hydrogel, referred to as a gelatin sheet in this study (2 x 2 cm), with bFGF (100 microg/site) (A) or without bFGF (B). 1, 2, 3, 5, 7 and 14 days after covering, mice were killed and an enzyme-linked immunosorbent assay (ELISA) was performed to estimate the concentration of bFGF in the plasma. In another experiment, each wound was covered with (A), (B) or a hydrogel dressing (control group, C) and the wound area was measured 1 or 2 weeks postoperatively with a computer planimeter. The histological parameters, as mentioned above, were assessed using a light microscope. Sustained release of bFGF from the gelatin sheet was observed and the gelatin sheet containing bFGF promoted neoepithelialization, granulation, neovascularization and wound closure. This gelatin sheet containing bFGF was concluded to be effective for wound healing and promising for clinical use.

Effects of bFGF incorporated into a gelatin sheet on wound healing. Publishing Authors By Initials

M Miyoshi,T Kawazoe,HH Igawa,Y Tabata,Y Ikada,S Suzuki,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration: wound healing research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration: wound healing research

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Effects of bFGF incorporated into a gelatin sheet on wound healing. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 16

Page Numbers: 893-907

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 2005

Effects of bFGF incorporated into a gelatin sheet on wound healing. Information

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LANGUAGE: eng

NlmUniqueID: 9007393

Effects of bFGF incorporated into a gelatin sheet on wound healing. Keywords Mesh Terms:

KEYWORDS: Wound Healing

MESH TERMS: physiology

Chemical & Substance for Abstract: Effects of bFGF incorporated into a gelatin sheet on wound healing. Information

Substance Name: Gelatin

Registry Number: 9000-70-8

Grant and Affiliation Information for Effects of bFGF incorporated into a gelatin sheet on wound healing.

AFFILIATION: Department of Plastic and Reconstructive Surgery, School of Medicine, Kagawa University, Kagawa, Japan. goji@kms.ac.jp

Country: Netherlands

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MEDLINETA: J Biomater Sci Polym Ed

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