Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions.

Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions. Research Abstract Details 

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Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions. Abstract Text:

Mengsheng Gao,Hancai Chen,Anatol Eberhard,Matthew R Gronquist,Jayne B Robinson,Mary Connolly,Max Teplitski,Barry G Rolfe,Wolfgang D Bauer,

Many behaviors in bacteria, including behaviors important to pathogenic and symbiotic interactions with eukaryotic hosts, are regulated by a mechanism called quorum sensing (QS). A "quorum-quenching" approach was used here to identify QS-regulated behaviors in the N-fixing bacterial symbiont Sinorhizobium meliloti. The AiiA lactonase from Bacillus produced in S. meliloti was shown to enzymatically inactivate S. meliloti's N-acyl homoserine lactone (AHL) QS signals, thereby disrupting normal QS regulation. Sixty proteins were differentially accumulated in the AiiA-producing strain versus the control in early log or early stationary phase cultures. Fifty-two of these QS-regulated proteins, with putative functions that include cell division, protein processing and translation, metabolite transport, oxidative stress, and amino acid metabolism, were identified by peptide mass fingerprinting. Transcription of representative genes was reduced significantly in the AiiA-producing strain, although the effects of AiiA on protein accumulation did not always correspond to effects on transcription. The QS signal-deficient strain was reduced significantly in nodule initiation during the first 12 h after inoculation onto Medicago truncatula host plants. The AiiA lactonase also was found to substantially inactivate two of the AHL mimic compounds secreted by M. truncatula. This suggests some structural similarity between bacterial AHLs and these mimic compounds. It also indicates that quorum quenching could be useful in identifying Sinorhizobium genes that are affected by such host QS mimics in planta.

Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions. Publishing Authors By Initials

M Gao,H Chen,A Eberhard,MR Gronquist,JB Robinson,M Connolly,M Teplitski,BG Rolfe,WD Bauer,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: symbiosis research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: symbiosis research

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Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular plant-microbe interactions : MPMI

VOLUME: 20

Page Numbers: 843-56

Journal Abbreviation: Mol. Plant Microbe Interact.

ISSN: 0894-0282

DAY: 3

MONTH: Jul

YEAR: 2007

Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9107902

Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions. Keywords Mesh Terms:

KEYWORDS: Symbiosis

MESH TERMS: metabolism

Chemical & Substance for Abstract: Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions. Information

Substance Name: N-acyl homoserine lactonase

Registry Number: EC 3.1.1.-

Grant and Affiliation Information for Effects of AiiA-mediated quorum quenching in Sinorhizobium meliloti on quorum-sensing signals, proteome patterns, and symbiotic interactions.

AFFILIATION: Department of Soil and Water Sciences, 2159 McCarty Hall A, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Gainesville 32611-0290, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM042893-14

ACRONYM: GM

MEDLINETA: Mol Plant Microbe Interact

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