Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells.

Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells. Research Abstract Details 

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Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells. Abstract Text:

Da-Huang Chen,Kuan-Tsu Wu,Chien-Jen Hung,Mingli Hsieh,Chuan Li,

Adenosine dialdehyde (AdOx) is an indirect methyltransferase inhibitor broadly used in cell culture to accumulate methyl-accepting proteins in hypomethylated states for in vitro protein methylation analyses. In this study we included a translation inhibitor, cycloheximide, in the AdOx treatment of HeLa cells. The methyl-accepting proteins disappeared in the double treatment, indicating that they were most likely newly synthesized in the AdOx incubation period. AdOx treatment could also be used in combination with in vivo methylation, another technique frequently used to study protein methylation. AdOx treatment prior to in vivo methylation accumulated methyl-accepting proteins for the labeling reaction. The continued presence of AdOx in the in vivo labeling period decreased the methylation of the majority of in vivo methyl-accepting polypeptides. The level and pattern of the in vivo methylated polypeptides did not change after a 12-h chase, supporting the notion that the methylated polypeptide as well as the methyl groups on the modified polypeptides are stable. On the other hand, methylarginine-specific antibodies detected limited but consistent reduction of the methylarginine-containing proteins in AdOx-treated samples compared to the untreated ones. Thus, AdOx treatment probably only blocked a small fraction of stable protein methylation. Overall, it is likely that base-stable methylation are formed soon after the synthesis of the polypeptide and remain stable after the modification.

Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells. Publishing Authors By Initials

DH Chen,KT Wu,CJ Hung,M Hsieh,C Li,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research

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Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 136

Page Numbers: 371-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 2004

Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells. Keywords Mesh Terms:

KEYWORDS: Trypsin

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells. Information

Substance Name: Proteasome Endopeptidase Complex

Registry Number: EC 3.4.25.1

Grant and Affiliation Information for Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells.

AFFILIATION: Department of Life Sciences, Chung Shan Medical University, Taichung, Taiwan, ROC.

Country: Japan

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MEDLINETA: J Biochem

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