Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal

Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal Abstract Text:

Cleber E Teixeira,Fernanda B M Priviero,R Clinton Webb,

We aimed to characterize the relaxation induced by the soluble guanylyl cyclase (sGC) stimulator 5-cyclopropyl-2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) and its pharmacological interactions with nitric oxide (NO) in the corpus cavernosum (CC) from wild-type (WT), endothelial nitric-oxide synthase (eNOS)(-/-), and neuronal (n)NOS(-/-) mice. The effect of BAY 41-2272 on superoxide formation and NADPH oxidase expression was also investigated. Tissues were mounted in myographs for isometric force recording. Enzyme immunoassay kits were used for cGMP determination. sGC activity was determined in the supernatant fractions of the cavernosal samples by the conversion of GTP to cGMP. Superoxide formation and expression of NADPH oxidase subunits were studied using the reduction of ferricytochrome c and Western blot analysis, respectively. BAY 41-2272 (0.01-10 microM) relaxed CC with pEC(50) values of 6.36 +/- 0.07 (WT), 6.27 +/- 0.06 (nNOS(-/-)), and 5.88 +/- 0.07 (eNOS(-/-)). The relaxations were accompanied by increases in cGMP levels. N(omega)-Nitro-L-arginine methyl ester inhibited BAY 41-2272-evoked responses in CC from WT and nNOS(-/-), but not eNOS(-/-).1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one reduced and sildenafil potentiated the relaxations induced by BAY 41-2272 in all groups. BAY 41-2272 enhanced NO (endogenous and exogenous)-induced relaxations in a concentration-dependent manner. Expression and activity of sGC was similar among the different groups. Superoxide formation was reduced by BAY 41-2272 (0.1-1 microM). The compound also inhibited p22(phox) and gp91(phox) expression induced by 9,11-dideoxy-11 alpha,9 alpha-epoxymethanoprostaglandin F(2 alpha (U46619). Our results demonstrated that sGC activation in the penis by BAY 41-2272 directly or via enhancement of NO effects may provide a novel treatment for erectile dysfunction, particularly in the event of an increased intrapenile oxidative stress.

Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal Publishing Authors By Initials

CE Teixeira,FB Priviero,RC Webb,

For similar inorganic chemicals: electrolytes: ions: anions: oxides: peroxides: superoxides research abstracts see: inorganic chemicals: electrolytes: ions: anions: oxides: peroxides: superoxides research

PUBMED ID PMID:

MEDLINE DATE:

Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of pharmacology and experimental thera

VOLUME: 322

Page Numbers: 1093-102

Journal Abbreviation: J. Pharmacol. Exp. Ther.

ISSN: 0022-3565

DAY: 27

MONTH: 06

YEAR: 2007

Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376362

Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal Keywords Mesh Terms:

KEYWORDS: Superoxides

MESH TERMS: analysis

Chemical & Substance for Abstract: Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal Information

Substance Name: soluble guanylyl cyclase

Registry Number: EC 4.6.1.2

Grant and Affiliation Information for Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal

AFFILIATION: Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, São Paulo, Brazil.

Country: United States

AGENCY: United States NHLBI

GRANT: HL74167

ACRONYM: HL

MEDLINETA: J Pharmacol Exp Ther

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Effects of 5-cyclopropyl-2-1-2-fluoro-benzyl-1H-pyrazolo3,4-bpyridine-3-ylpyrimidin-4-ylamine BAY 41-2272 on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal Related Publications

• A dynamic model of thoracic differentiation for the control of turning in the stick insect.
• A low-power, LED-based, high-brightness anomaloscope.
• A spatial and temporal comparison of hemodynamic signals measured using optical and functional magnetic resonance imaging during activation in the human primary visual cortex.
• A system for success: BMC Systems Biology, a new open access journal.
• ARBOVIRUS INFECTIONS IN SARAWAK: OBSERVATIONS ON THE MOSQUITOES.
• C7 decancellisation closing wedge osteotomy for the correction of fixed cervico-thoracic kyphosis.
• Comments on D. Blaze-Temple's 'Drug testing in the Australian workplace: an overview of the issues'.
• Determination of adenosine effects and adenosine receptors in murine corpus cavernosum.
• Diagnostic aids for the detection of urine in the equine ejaculate.
• Disease mongering -- a challenge for everyone involved in healthcare.
• Dynamics of equilibrium structural fluctuations of apomyoglobin measured by fluorescence correlation spectroscopy.
• Effects of 5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]pyrimidin-4-ylamine (BAY 41-2272) on smooth muscle tone, soluble guanylyl cyclase activity, and NADPH oxidase activity/expression in corpus cavernosum from wild-type, neuronal
• Effects of ractopamine and protein source on growth performance and carcass characteristics of feedlot heifers.
• Enantiomeric drugs-separation by chromatography or by specific immunoassay.
• HDL cholesterol transport during inflammation.
• Interferometry from a scattering medium.
• Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles.
• Level-set algorithm for the reconstruction of functional activation in near-infrared spectroscopic imaging.
• Line-scanning laser ophthalmoscope.
• Magnetic resonance microimaging and numerical simulations of velocity fields inside enlarged flow cells used for coupled NMR microseparations.
• Modeling biological fluorescence emission spectra using Lorentz line shapes and nonlinear optimization.
• Nuclear magnetic resonance of mass-limited samples using small RF coils.
• Outcome of Henoch-Schönlein purpura nephritis treated with long-term immunosuppression.
• Spatial and temporal hemodynamic study of human primary visual cortex using simultaneous functional MRI and diffuse optical tomography.
• Study of and Correction for Intra-fraction Tumour Motion.
• Succinic acid production from wheat using a biorefining strategy.
• The costs of key worker support for disabled children and their families.
• The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks.
• The three official language versions of the Declaration of Helsinki: what's lost in translation?
• Validation of internal controls for gene expression analysis in the intestine of rats infected with Hymenolepis diminuta.