Effect of transforming growth factor-beta(2) on extracellular matrix synthesis in bovine trabecular meshwork cells.

Effect of transforming growth factor-beta(2) on extracellular matrix synthesis in bovine trabecular meshwork cells. Research Abstract Details 

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Effect of transforming growth factor-beta(2) on extracellular matrix synthesis in bovine trabecular meshwork cells. Abstract Text:

Yang Cao,Houren Wei,Ying Zhang,Banghong Da,Yinping Lu,

OBJECTIVE: To investigate the effect of transforming growth factor-beta(2) (TGF-beta(2)) in human aqueous humor on extracellular matrix synthesis in cultured bovine trabecular meshwork cells. METHODS: Cultured 3-5 passage bovine trabecular meshwork cells were divided into control group and experimental group. After treated with 0 ng/L (control), 0.32 x 10(3) ng/L, 1.00 x 10(3) ng/L, 3.20 x 10(3) ng/L TGF-beta(2) for 48 h, collagen, fibronectin and hyaluronic acid synthesis in bovine trabecular meshwork cells were examined respectively by (3)H-proline incorportion and liquid scintillation technique, enzyme linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). RESULTS: In comparison with the control group, TGF-beta(2) significantly promoted the synthesis of collagen at 0.32 x 10(3) ng/L (P < 0.05), 1.00 x 10(3) ng/L (P < 0.01), 3.20 x 10(3) ng/L (P < 0.01), (3)H-proline incorporation increased in a concentration-dependent manner; and 1.00 x 10(3) ng/L (P < 0.05), 3.20 x 10(3) ng/L (P < 0.01) TGF-beta(2) significantly promoted the synthesis of fibronectin in cultured bovine trabecular meshwork cells. 1.00 x 10(3) ng/L (P < 0.01) and 3.20 x 10(3) ng/L (P < 0.01) TGF-beta(2) significantly inhibited the synthesis of hyaluronic acid in the cultured cells. CONCLUSION: The data suggest that TGF-beta(2) play important roles in extracellular matrix age-related changes of normal trabecular meshwork and abnormal deposition of the extracellular matrix in the trabecular meshwork, especially the juxtacanalicular tissue of primary open-angle glaucoma patients.

Effect of transforming growth factor-beta(2) on extracellular matrix synthesis in bovine trabecular meshwork cells. Publishing Authors By Initials

Y Cao,H Wei,Y Zhang,B Da,Y Lu,

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Effect of transforming growth factor-beta(2) on extracellular matrix synthesis in bovine trabecular meshwork cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: [Zhonghua yan ke za zhi] Chinese journal of ophtha

VOLUME: 38

Page Numbers: 429-32

Journal Abbreviation: Zhonghua Yan Ke Za Zhi

ISSN: 0412-4081

DAY: 4

MONTH: Jul

YEAR: 2002

Effect of transforming growth factor-beta(2) on extracellular matrix synthesis in bovine trabecular meshwork cells. Information

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LANGUAGE: eng

NlmUniqueID: 16210540

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AFFILIATION: Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China (Email: ytsao@sohu.com)

Country: China

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MEDLINETA: Zhonghua Yan Ke Za Zhi

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