Effect of superoxide dismutase on the development of preimplantation mouse embryos.

Effect of superoxide dismutase on the development of preimplantation mouse embryos. Research Abstract Details 

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Effect of superoxide dismutase on the development of preimplantation mouse embryos. Abstract Text:

Y S Chun,J H Kim,H T Lee,K S Chung,

The role of superoxide dismutase (SOD) was tested on preimplantation development of mouse embryos in vitro. The presence of SOD in ovarian antral follicles and in oviductal and uterine secretions was also investigated. Zygotes from superovulated ICR female mice were cultured in modified Whittingham's T6 medium supplemented with SOD (0 to 370 U) or EDTA (100 muM) at 37 degrees C under 5% CO(2) in air. Supplementation of SOD (370 U) significantly promoted the development of zygotes to the blastocyst stage (45%) as compared to that of the controls (1.4%). This favorable effect of SOD was comparable to that of EDTA and completely suppressed by anti-SOD antibody. Blastocysts cultured with SOD consisted of 78.2+/-10.4 blastomeres and possessed as many blastomeres as those (81.6+/-9.3) developing in vivo; blastocysts cultured with EDTA had significantly fewer blastomeres (42.6+/-13.7). These findings suggest that SOD protects embryos against oxidative insults and that it can be an effective substitute for EDTA for supporting mouse embryo development in vitro. The SOD activity was detected in 3 different lumina from mouse reproductive organs, and SOD was identified as a cytosolic Cu,Zn-SOD on photochemically stained polyacrylamide gels. Our results suggest that oxidative injury may be responsible for developmental retardation of preimplantation-stage mouse embryos in vitro and that Cu,Zn-SOD may play a crucial role in protecting embryos against oxygen toxicity in vivo as well as in vitro.

Effect of superoxide dismutase on the development of preimplantation mouse embryos. Publishing Authors By Initials

YS Chun,JH Kim,HT Lee,KS Chung,

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Effect of superoxide dismutase on the development of preimplantation mouse embryos. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 41

Page Numbers: 511-20

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Feb

YEAR: 1994

Effect of superoxide dismutase on the development of preimplantation mouse embryos. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Effect of superoxide dismutase on the development of preimplantation mouse embryos.

AFFILIATION: Department of Animal Science, Animal Resources Research Center Kon-Kuk University, 93-1 Mojin-Dong, Seongdong-Ku, Seoul 133-701, Korea.

Country: United States

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MEDLINETA: Theriogenology

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