Effect of retinoblastoma gene on human osteosarcoma cell line OS732.

Effect of retinoblastoma gene on human osteosarcoma cell line OS732. Research Abstract Details 

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Effect of retinoblastoma gene on human osteosarcoma cell line OS732. Abstract Text:

Hui Qiao,Jushi Qiu,Yang Li,Meng Zhang,

OBJECTIVE: To investigate the effect of growth suppression and gap junctional intercellular communication (GJIC) on osteosarcoma cell line OS732 transduced with the exogenous N-terminal truncated retinoblastoma (Rb) gene. METHODS: Recombinant eukaryotic expression plasmid harbouring 1.65 kb Rb gene was constructed and transduced into OS732 cells. Rb mRNA expression was detected by RT-PCR and Northern blot. The cell count, cell cycle analysis and soft agar colony formation test were used to observe growth features. RT-PCR and Lucifer yellow dye transfer were carried out to measure GJIC. RESULTS: Both exogenous and endogenous Rb gene could be detected in OS732 cell transduced with the N-terminal truncated Rb gene. Compared with the parental cells, change in morphology of Rb-transduced cells were found; growth rate decreased; amount of colony formation reduced; cell cycle arrested at G(0)/G(1) checkpoint; Connexin43 expression and GJIC enhanced. CONCLUSIONS: The malignant phenotype of OS732 is inhibited and GJIC is increased by transduction of N-terminal truncated Rb gene.

Effect of retinoblastoma gene on human osteosarcoma cell line OS732. Publishing Authors By Initials

H Qiao,J Qiu,Y Li,M Zhang,

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Effect of retinoblastoma gene on human osteosarcoma cell line OS732. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua bing li xue za zhi Chinese journal of pat

VOLUME: 31

Page Numbers: 236-9

Journal Abbreviation: Zhonghua Bing Li Xue Za Zhi

ISSN: 0529-5807

DAY: 11

MONTH: Jun

YEAR: 2002

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LANGUAGE: eng

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AFFILIATION: Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China (Email: qiaohui99@163.net)

Country: China

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MEDLINETA: Zhonghua Bing Li Xue Za Zhi

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