Effect of oxygen concentration in the incubator's gas phase on the development of cultured preimplantation rabbit embryos.

Effect of oxygen concentration in the incubator's gas phase on the development of cultured preimplantation rabbit embryos. Research Abstract Details 

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Effect of oxygen concentration in the incubator's gas phase on the development of cultured preimplantation rabbit embryos. Abstract Text:

A Lindenau,B Fischer,

Development of rabbit preimplantation embryos cultured under 20, 5 or 1% oxygen was studied. Three-day-old morulae were cultured in a protein-free medium (BSM II supplemented with 5 mg PVP/ml medium) for 24 and 48 h. Embryonic development was evaluated by gross morphology and by incorporation of tritiated thymidine as an indicator of cell proliferation. The lower oxygen concentrations yielded significantly better embryo development at 24 and at 48 h than the 20% concentration. There was no significant difference in development between 5% and 1% oxygen. Addition of the radical scavanger superoxiddismutase (SOD), either alone or in combination with catalase or reduced glutathione, did not improve embryo development even in the 20% oxygen group. Our data suggest the need to reduce in vitro oxygen levels from 20% to more physiological concentrations.

Effect of oxygen concentration in the incubator's gas phase on the development of cultured preimplantation rabbit embryos. Publishing Authors By Initials

A Lindenau,B Fischer,

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Effect of oxygen concentration in the incubator's gas phase on the development of cultured preimplantation rabbit embryos. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 41

Page Numbers: 889-98

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: 05

YEAR: 1994

Effect of oxygen concentration in the incubator's gas phase on the development of cultured preimplantation rabbit embryos. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Effect of oxygen concentration in the incubator's gas phase on the development of cultured preimplantation rabbit embryos.

AFFILIATION: Institut für Anatomie und Reproduktionsbiologie der RWTH Aachen D-52057 Aachen, Germany.

Country: United States

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MEDLINETA: Theriogenology

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