Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli.

Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli. Research Abstract Details 

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Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli. Abstract Text:

S C Lee,P O Olins,

The effect of overexpression of the heat shock chaperone genes dnaK and groESL on heterologous protein production in Escherichia coli was examined, using a set of related human procollagenase proteins. A diverse range of effects on protein solubility, secretion, and accumulation was observed, and these effects were highly dependent on the particular chaperone/procollagenase pairing involved. Both chaperones caused a large increase in the apparent solubility of a fusion of the LamB signal peptide to procollagenase. GroESL had no effect on the accumulation of mature (secreted) procollagenase, while DnaK suppressed secretion considerably. In the absence of a signal peptide, overexpression of either chaperone resulted in a dramatic increase in both solubility and accumulation of procollagenase. The 10-fold increase in accumulation was associated with an increase in in vivo protein half-life.

Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli. Publishing Authors By Initials

SC Lee,PO Olins,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 267

Page Numbers: 2849-52

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 15

MONTH: Feb

YEAR: 1992

Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli. Information

Substance Name: dnaK protein, E coli

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Effect of overproduction of heat shock chaperones GroESL and DnaK on human procollagenase production in Escherichia coli.

AFFILIATION: Monsanto Corporate Research, Monsanto Company, St. Louis, Missouri 63198.

Country: UNITED STATES

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MEDLINETA: J Biol Chem

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