Effect of high-density lipoproteins on cholesterol efflux and esterification in lipid-enriched human skin fibroblasts.

Effect of high-density lipoproteins on cholesterol efflux and esterification in lipid-enriched human skin fibroblasts. Research Abstract Details 

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Effect of high-density lipoproteins on cholesterol efflux and esterification in lipid-enriched human skin fibroblasts. Abstract Text:

S A Evensen,J P Blomhoff,E Nilsen,R Holme,H Letnes,

The ability of high-density lipoprotein (HDL) to reduce the cholesterol content was studied in cultured fibroblasts enriched with cholesterol esters. Incubation of cholesterol-enriched cells with HDL in a final concentration of 1 g protein/l for 24 h reduced the total and esterified cholesterol content by 23% as compared with control fibroblasts incubated with albumin. Similar cholesterol efflux was obtained with HDL isolated from lecithin:cholesterol acyltransferase (LCAT)-deficient plasma. The HDL3 subfraction isolated by rate-zonal ultracentrifugation contained the major part of the cholesterol-depleting effect. HDL or HDL3 decreased CoA:cholesterol acyltransferase (ACAT) activity to 5% of the level found in control fibroblasts within 8 h of incubation. These findings suggest that ACAT activity is sensitive to a pool of intracellular cholesterol, which can be mobilized by the addition of HDL to the culture medium, and that ACAT activity is a useful measure of cholesterol efflux from cultured fibroblasts.

Effect of high-density lipoproteins on cholesterol efflux and esterification in lipid-enriched human skin fibroblasts. Publishing Authors By Initials

SA Evensen,JP Blomhoff,E Nilsen,R Holme,H Letnes,

For similar integumentary system: skin research abstracts see: integumentary system: skin research

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Effect of high-density lipoproteins on cholesterol efflux and esterification in lipid-enriched human skin fibroblasts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Scandinavian journal of gastroenterology. Suppleme

VOLUME: 107

Page Numbers: 61-6

Journal Abbreviation: Scand. J. Gastroenterol. Suppl

ISSN: 0085-5928

DAY: 13

MONTH: 02

YEAR: 1985

Effect of high-density lipoproteins on cholesterol efflux and esterification in lipid-enriched human skin fibroblasts. Information

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LANGUAGE: eng

NlmUniqueID: 437034

Effect of high-density lipoproteins on cholesterol efflux and esterification in lipid-enriched human skin fibroblasts. Keywords Mesh Terms:

KEYWORDS: Skin

MESH TERMS: cytology

Chemical & Substance for Abstract: Effect of high-density lipoproteins on cholesterol efflux and esterification in lipid-enriched human skin fibroblasts. Information

Substance Name: Phosphatidylcholine-Sterol O-Acyltransfe

Registry Number: EC 2.3.1.43

Grant and Affiliation Information for Effect of high-density lipoproteins on cholesterol efflux and esterification in lipid-enriched human skin fibroblasts.

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Country: NORWAY

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MEDLINETA: Scand J Gastroenterol Suppl

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