Effect of glycerol level in Tris-basek extender and equilibration period on quality of frozen goat semen.

Effect of glycerol level in Tris-basek extender and equilibration period on quality of frozen goat semen. Research Abstract Details 

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Effect of glycerol level in Tris-basek extender and equilibration period on quality of frozen goat semen. Abstract Text:

B C Deka,A R Rao,

Twenty ejaculates, 4 from each of 5 native goats, were collected using an artificial vagina, and the effects of glycerol level (4, 6.4 and 9 %) and the equilibration period (1, 3 and 5 h) were studied by split-sample technique. The extender used was Tris egg yolk citric acid fructose glycerol extender. The semen was frozen in 0.5-ml French straws by exposure for 10 min to liquid nitrogen vapor, 5 cm above the liquid nitrogen level. After 14 h of storage in liquid nitrogen, the straws were thawed in water at 37 degrees C for 12 - 15 sec. The percentage of progressively motile sperm (PPM) and the percentage of damaged acrosomes (PDA) were studied after equilibration and after thawing. The mean PPM after thawing was found to be 64.0 +/- 0.90, 66.92 +/- 0.54 and 63.65 +/- 1.07 when semen was frozen with 4, 6.4 and 9 % glycerol and 61.48 +/- 0.81, 65.05 +/- 0.78 and 68.03 +/- 0.87 in 1-, 3- and 5-h equilibrated semen, respectively. The mean PDA after thawing was 7.12 +/- 0.88, 8.23 +/- 0.76 and 10.58 +/- 0.84 when semen was frozen with 4, 6.4 and 9 % glycerol and 7.0 +/- 0.74, 9.0 +/- 0.95 and 9.93 +/- 0.81 in 1-, 3- and 5-h equilibrated semen, respectively. Both PPM and PDA differed significantly (P<0.01) between glycerol levels, between equilibration periods and between stages (after equilibration and after thawing). The PPM also differed significantly due to equilibration period x stage interaction (P<0.01) and glycerol level x stage interaction (P<0.05). The PDA did not differ significantly due to interactions. When the differences between pairs of means were tested by least significant difference, it was found that after equilibration PPM was not significantly affected by either glycerol level or equilibration period, while after thawing, it was significantly higher (P<0.05) for 6.4 % glycerol and 5-h equilibrated semen than for 4 or 9 % glycerol and 1- or 3-h equilibrated semen, respectively. The PDA was lower with 4 % glycerol and 1-h equilibrated semen.

Effect of glycerol level in Tris-basek extender and equilibration period on quality of frozen goat semen. Publishing Authors By Initials

BC Deka,AR Rao,

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Effect of glycerol level in Tris-basek extender and equilibration period on quality of frozen goat semen. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 26

Page Numbers: 231-8

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Aug

YEAR: 1986

Effect of glycerol level in Tris-basek extender and equilibration period on quality of frozen goat semen. Information

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LANGUAGE: eng

NlmUniqueID: 421510

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Grant and Affiliation Information for Effect of glycerol level in Tris-basek extender and equilibration period on quality of frozen goat semen.

AFFILIATION: College of Veterinary Science Tirupati, Andhra Pradesh, India.

Country: United States

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MEDLINETA: Theriogenology

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