Effect of follicle stimulation hormone and luteinizing hormone on cumulus cell expansion and in vitro nuclear maturation of canine oocytes.

Effect of follicle stimulation hormone and luteinizing hormone on cumulus cell expansion and in vitro nuclear maturation of canine oocytes. Research Abstract Details 

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Effect of follicle stimulation hormone and luteinizing hormone on cumulus cell expansion and in vitro nuclear maturation of canine oocytes. Abstract Text:

H-S Lee,Y-I Seo,X-J Yin,S-G Cho,S-S Lee,N-H Kim,S-K Cho,I-K Kong,H-S Lee,Y-I Seo,X-J Yin,S-G Cho,S-S Lee,N-H Kim,S-K Cho,I-K Kong,H-S Lee,Y-I Seo,X-J Yin,S-G Cho,S-S Lee,N-H Kim,S-K Cho,I-K Kong,

In general, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in the regulation of cumulus cell expansion and oocyte maturation. We investigated the effects of supplementation of FSH or LH in in vitro maturation (IVM) medium on the incidence of cumulus cell expansion and nuclear maturation in canine oocytes. Cumulus-oocyte complexes (COCs) were cultured in TCM-199 supplemented with 10% foetal bovine serum (FBS), 1 mg/ml cysteine, 0.2 mm pyruvic acid and different concentrations of FSH or LH (control, 0.5, 5 or 50 mug/ml) at 38.5 degrees C, 5% CO(2) in air for 72 h. The cumulus cell expansion was measured by microscopic visualization, and nuclear maturation of denuded oocytes was determined by staining with 10 mug/ml Hoechst33342 for 30 min. The cumulus cell expansion in the 5 mug/ml FSH group (397.2 +/- 64.3 mum) was significantly higher than those in the control, 0.5, and 50 mug/ml FSH groups (168.3 +/- 19.1, 286.0 +/- 69.7 and 300.0 +/- 84.3 mum, respectively; p < 0.05). However, there was no difference in cumulus cell expansion among the control, 0.5, 5 and 50 mug/ml LH groups (165.6 +/- 20.2, 160 +/- 26.5, 172 +/- 20.5 and 168 +/- 23.1 mum, respectively; p > 0.05). After 72 h of IVM, the proportion of nuclear development to the MI-MII stage in the 0.5 mug/ml FSH group (15.1%) was higher than those in the control, 0.5 and 50 mug/ml FSH groups (0.9%, 6.5% and 8.0%, respectively; p < 0.05). However, there was no significant difference in nuclear maturation to the MI-MII stage among control, 0.5, 5 and 50 mug/ml LH groups (4.6%, 2.3%, 5.4% and 8.6%, respectively; p > 0.05). This study indicated that a FSH supplement in IVM medium can increase cumulus cell expansion and nuclear maturation, while the nuclear maturation rate remained low. Further studies are required to improve the nuclear development to the MI-MII stages in canine oocytes.

Effect of follicle stimulation hormone and luteinizing hormone on cumulus cell expansion and in vitro nuclear maturation of canine oocytes. Publishing Authors By Initials

HS Lee,YI Seo,XJ Yin,SG Cho,SS Lee,NH Kim,SK Cho,IK Kong,HS Lee,YI Seo,XJ Yin,SG Cho,SS Lee,NH Kim,SK Cho,IK Kong,HS Lee,YI Seo,XJ Yin,SG Cho,SS Lee,NH Kim,SK Cho,IK Kong,

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Effect of follicle stimulation hormone and luteinizing hormone on cumulus cell expansion and in vitro nuclear maturation of canine oocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Reproduction in domestic animals = Zuchthygiene

VOLUME: 42

Page Numbers: 561-5

Journal Abbreviation: Reprod. Domest. Anim.

ISSN: 0936-6768

DAY: 2

MONTH: Dec

YEAR: 2007

Effect of follicle stimulation hormone and luteinizing hormone on cumulus cell expansion and in vitro nuclear maturation of canine oocytes. Information

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LANGUAGE: eng

NlmUniqueID: 9015668

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Grant and Affiliation Information for Effect of follicle stimulation hormone and luteinizing hormone on cumulus cell expansion and in vitro nuclear maturation of canine oocytes.

AFFILIATION: Department of Animal Science & Technology, Sunchon National University, Suncheon, South Korea.

Country: Germany

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MEDLINETA: Reprod Domest Anim

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