Effect of estrogen on the expression of luteinizing hormone-human chorionic gonadotropin receptor messenger ribonucleic Acid in cultured rat granulosa cells.

Effect of estrogen on the expression of luteinizing hormone-human chorionic gonadotropin receptor messenger ribonucleic Acid in cultured rat granulosa cells. Research Abstract Details 

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Effect of estrogen on the expression of luteinizing hormone-human chorionic gonadotropin receptor messenger ribonucleic Acid in cultured rat granulosa cells. Abstract Text:

Sadatomo Ikeda,Kazuto Nakamura,Kayoko Kogure,Yuki Omori,Soichi Yamashita,Kazuko Kubota,Tetsuya Mizutani,Kaoru Miyamoto,Takashi Minegishi,

Estrogen has been considered to enhance FSH actions in the ovary, including the induction of the LH receptor (LHR). In this study, we elucidated the mechanism underlying the effect of estrogen on the induction of LHR by FSH in rat granulosa cells. Estradiol clearly enhanced the FSH-induced LHR mRNA increase in a time- and dose-dependent manner, with a maximum increase of approximately 3.5-fold at 72 h, compared with the level of LHR mRNA solely induced by FSH. We then investigated whether the effect of estrogen on LHR mRNA was due to increased transcription and/or altered mRNA stability. A luciferase assay with the plasmid containing the LHR 5'-flanking region did not show that estradiol increased the promoter activity induced by FSH. In contrast, the decay curves for LHR mRNA showed a significant increase in half-life with FSH and estradiol, suggesting that the increased stability of LHR mRNA is at least responsible for the regulation of LHR mRNA by estrogen. Recently mevalonate kinase (Mvk) was identified as a trans-factor that binds to LHR mRNA and alters LHR mRNA stability in the ovary. We found that estradiol, with FSH, decreased Mvk mRNA levels in rat granulosa cell culture, resulting in up-regulation of LHR mRNA that was inversely correlated to Mvk mRNA expression. Furthermore, the augmentation of FSH-induced LHR expression in the presence of estrogen was erased with the overexpression of Mvk by transient transfection. Taken together, these data indicate that LHR mRNA is up-regulated due to increased stability when estrogen negatively controls Mvk.

Effect of estrogen on the expression of luteinizing hormone-human chorionic gonadotropin receptor messenger ribonucleic Acid in cultured rat granulosa cells. Publishing Authors By Initials

S Ikeda,K Nakamura,K Kogure,Y Omori,S Yamashita,K Kubota,T Mizutani,K Miyamoto,T Minegishi,

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Effect of estrogen on the expression of luteinizing hormone-human chorionic gonadotropin receptor messenger ribonucleic Acid in cultured rat granulosa cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Endocrinology

VOLUME: 149

Page Numbers: 1524-33

Journal Abbreviation: Endocrinology

ISSN: 0013-7227

DAY: 3

MONTH: 01

YEAR: 2008

Effect of estrogen on the expression of luteinizing hormone-human chorionic gonadotropin receptor messenger ribonucleic Acid in cultured rat granulosa cells. Information

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LANGUAGE: eng

NlmUniqueID: 375040

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Grant and Affiliation Information for Effect of estrogen on the expression of luteinizing hormone-human chorionic gonadotropin receptor messenger ribonucleic Acid in cultured rat granulosa cells.

AFFILIATION: Department of Gynecology and Reproductive Medicine, Gunma University Graduate School of Medicine, Gunma 371-8511, Japan. nkazuto@med.gunma-u.ac.jp.

Country: United States

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MEDLINETA: Endocrinology

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