Effect of ENA modification of triplex-forming oligonucleotide on pyrimidine motif triplex formation.

Effect of ENA modification of triplex-forming oligonucleotide on pyrimidine motif triplex formation. Research Abstract Details 

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Effect of ENA modification of triplex-forming oligonucleotide on pyrimidine motif triplex formation. Abstract Text:

Hidetaka Torigoe,Nobuyuki Nagasawa,

We examined the effect of 2'-O,4'-C-ethylene bridged nucleic acid (ENA) backbone modification of triplex-forming oligonucleotide (TFO) on the pyrimidine motif triplex formation at neutral pH, a condition where pyrimidine motif triplexes are unstable. The binding constant of the pyrimidine motif triplex formation at pH 6.8 with ENA-modified TFO was 20 times larger than that observed with unmodified TFO. The number and position of the ENA modification introduced into the TFO did not significantly affect the magnitude of the increase in the binding constant. The consideration of the observed thermodynamic parameters suggested that the increased rigidity itself of the ENA-modified TFO in the free state relative to the unmodified TFO may enable the significant increase in the binding constant at neutral pH. The present results certainly support the idea that the ENA backbone modification of TFO could be a key chemical modification and may eventually lead to progress in therapeutic applications of the antigene strategy in vivo.

Effect of ENA modification of triplex-forming oligonucleotide on pyrimidine motif triplex formation. Publishing Authors By Initials

H Torigoe,N Nagasawa,

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Effect of ENA modification of triplex-forming oligonucleotide on pyrimidine motif triplex formation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nucleic acids symposium series (2004)

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Page Numbers: 161-2

Journal Abbreviation: Nucleic Acids Symp Ser (Oxf)

ISSN: 1746-8272

DAY: 21

MONTH: 11

YEAR: 2007

Effect of ENA modification of triplex-forming oligonucleotide on pyrimidine motif triplex formation. Information

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LANGUAGE: eng

NlmUniqueID: 101259965

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AFFILIATION: Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan. htorigoe@rs.kagu.tus.ac.jp

Country: England

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MEDLINETA: Nucleic Acids Symp Ser (Oxf)

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