Effect of electrostatic interactions on binding and retention of DNA oligomers to PNA liposomes assessed by FRET measurements.

Effect of electrostatic interactions on binding and retention of DNA oligomers to PNA liposomes assessed by FRET measurements. Research Abstract Details 

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Effect of electrostatic interactions on binding and retention of DNA oligomers to PNA liposomes assessed by FRET measurements. Abstract Text:

Bruno F Marques,James W Schneider,

A FRET-based method is used to observe the desorption of di-alkyl peptide nucleic acid amphiphiles (PNAA) from liposomes occurring on binding of complementary DNA oligomers. PNA liposomes were prepared containing fluorescein-labeled PNAA and rhodamine-labeled dipalmitoylphosphoethanolamine (DPPE). These liposomes showed efficient energy transfer from the fluorescein to rhodamine, with an average donor-to-acceptor distance of 5.91nm. In low-ionic-strength buffer (50mM Tris-HCl, pH 8.0), the FRET signal was maintained in the presence of a stoichiometric amount of 10- and 20-mers DNA complements, but the signal attenuated for 40-mer complements, indicating that DNA first binds the PNAA before the PNAA/DNA duplex desorbs from the lipid bilayer. The FRET signal was maintained in the presence of 10-, 20-, 40-, and 60-mer DNA in high ionic-strength buffer, showing that the driving force for the desorption is electrostatic repulsion between the bound DNA oligomer and the liposome surface. This conclusion is corroborated by comparison of the PNA/DNA binding energy, the energy of adsorption of the di-alkyl PNAA to the lipid bilayer, and a calculation of the DNA/lipid bilayer electrostatic repulsion using the linearized Poisson-Boltzmann equation.

Effect of electrostatic interactions on binding and retention of DNA oligomers to PNA liposomes assessed by FRET measurements. Publishing Authors By Initials

BF Marques,JW Schneider,

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Effect of electrostatic interactions on binding and retention of DNA oligomers to PNA liposomes assessed by FRET measurements. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Colloids and surfaces. B, Biointerfaces

VOLUME: 53

Page Numbers: 1-8

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ISSN: 0927-7765

DAY: 16

MONTH: 07

YEAR: 2006

Effect of electrostatic interactions on binding and retention of DNA oligomers to PNA liposomes assessed by FRET measurements. Information

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LANGUAGE: eng

NlmUniqueID: 9315133

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Grant and Affiliation Information for Effect of electrostatic interactions on binding and retention of DNA oligomers to PNA liposomes assessed by FRET measurements.

AFFILIATION: Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213-3890, Unites States.

Country: Netherlands

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MEDLINETA: Colloids Surf B Biointerfaces

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