The carbohydrate-binding protein, discoidin, was synthesized from Dictyostelium discoideum NC-4 grown on a Bacto peptone (Difco) containing medium together with bacteria. When the cells were transferred by serial passage to a Proteose peptone (Daigo)-containing medium, the productivity of discoidin was reduced to a negligible amount, but the cells formed aggregates, and another protein which binds with Sepharose 4B was detected by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. We conclude that discoidin synthesis is already regulated during growth, and even when the amount of this protein is nil or negligible, the cells still retain aggregate-forming ability.
Effect of different growth media on the synthesis of carbohydrate-Binding protein from Dictyostelium discoideum NC-4. Publishing Authors By Initials
Effect of different growth media on the synthesis of carbohydrate-Binding protein from Dictyostelium discoideum NC-4. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biochemistry
VOLUME: 87
Page Numbers: 333-8
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: Jan
YEAR: 1980
Effect of different growth media on the synthesis of carbohydrate-Binding protein from Dictyostelium discoideum NC-4. Information
Number of References:
LANGUAGE: eng
NlmUniqueID: 376600
Effect of different growth media on the synthesis of carbohydrate-Binding protein from Dictyostelium discoideum NC-4. Keywords Mesh Terms:
KEYWORDS: Protozoan Proteins
MESH TERMS: metabolism
Chemical & Substance for Abstract: Effect of different growth media on the synthesis of carbohydrate-Binding protein from Dictyostelium discoideum NC-4. Information
Substance Name: discoidins
Registry Number: 0
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MEDLINETA: J Biochem
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