Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro.

Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro. Research Abstract Details 

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Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro. Abstract Text:

PURPOSE: To assess the influence of different concentrations of recombinant human leukemia inhibitory factor (LIF) on the in vitro development of mouse embryos. METHODS: The 2- to 4-cell embryos of CB6F1 mice were cultured in the human tubal fluid (HTF) media containing different concentrations of LIF. Mouse embryos were divided into seven groups: (1) HTF; (2) 1500 IU/ml LIF; (3) 1000 IU/ml LIF; (4) 750 IU/ml LIF; (5) 500 IU/ml LIF; (6) 250 IU/ml LIF; (7) 125 IU/ml LIF. The embryonic numbers of different stages including 5-8 cell, 9-16 cell, morula, blastocyst, and hatching blastocyst were recorded. RESULTS: The percentage of early embryo stage (2-cell embryos to 6- to 16-cell stages) in all groups were nonsignificantly different. There were higher formation rates of preimplantation embryos (morula to hatching blastocyst) in groups 2, 3, 4, and 5 than in groups 1, 6 and 7. CONCLUSIONS: LIF has positive effects on preimplantation embryo development and has nonsignificant influence on the early embryo development. The lowest concentration of LIF which could provide the optimal embryo development is 500 IU/ml.

Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro. Publishing Authors By Initials

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Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 17

Page Numbers: 352-5

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Jul

YEAR: 2000

Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro.

AFFILIATION: Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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