Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium.

Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium. Research Abstract Details 

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Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium. Abstract Text:

Tobias ,Christoph Wittmann,Wei Wang,Ezequiel Franco-Lara,Dieter Jahn,Wolf-Dieter Deckwer,Tobias ,Christoph Wittmann,Wei Wang,Ezequiel Franco-Lara,Dieter Jahn,Wolf-Dieter Deckwer,Tobias ,Christoph Wittmann,Wei Wang,Ezequiel Franco-Lara,Dieter Jahn,Wolf-Dieter Deckwer,

The recombinant Bacillus megaterium strain WH323 was employed for the inducible production and secretion of recombinant Thermobifida fusca hydrolase (TFH). Continuous cultivations were carried out in a chemostat using either glucose or pyruvate as sole carbon source. A remarkable increase of produced TFH was detected for the pyruvate-dependent cultivation compared to glucose-dependent growth. Estimation of intracellular carbon fluxes through the central metabolism for both growth conditions using (13)C-labelled substrates revealed noticeable changes of the fluxes through the tricarboxylic acid cycle, the pentose phosphate pathway and around the pyruvate node when protein production was induced. With pyruvate as sole carbon source the observed alterations of the fluxes yielded an increased production of ATP and NADPH both required for the anabolism. Additionally, the analysis of the corresponding secretome revealed significantly reduced amounts of extracellular proteases in the medium compared to glucose-grown cultivations. Thus, pyruvate-dependent chemostat cultivation was identified as a favourable condition for production and secretion of recombinant TFH using B. megaterium as production host.

Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium. Publishing Authors By Initials

T ,C Wittmann,W Wang,E Franco-Lara,D Jahn,WD Deckwer,T ,C Wittmann,W Wang,E Franco-Lara,D Jahn,WD Deckwer,T ,C Wittmann,W Wang,E Franco-Lara,D Jahn,WD Deckwer,

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Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biotechnology

VOLUME: 132

Page Numbers: 385-94

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ISSN: 0168-1656

DAY: 7

MONTH: 08

YEAR: 2007

Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium. Information

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LANGUAGE: eng

NlmUniqueID: 8411927

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Grant and Affiliation Information for Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium.

AFFILIATION: Technical University Braunschweig/HZI-Helmholtz Zentrum für Infektionsforschung, Institute of Biochemical Engineering, Inhoffenstrasse 7, D-38124 Braunschweig, Germany.

Country: Netherlands

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MEDLINETA: J Biotechnol

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