Effect of castration on male rabbit lower urinary tract tissue enzymes.

Effect of castration on male rabbit lower urinary tract tissue enzymes. Research Abstract Details 

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Effect of castration on male rabbit lower urinary tract tissue enzymes. Abstract Text:

Yung-Shun Juan,Bulent Onal,Samuel Broadaway,Julia Cosgrove,Robert E Leggett,Catherine Whitbeck,Elise De,Rebekah Sokol,Robert M Levin,

OBJECTIVES: The influence of testosterone on the prostate and corpus cavernosum have been studied extensively. However, the influence of testosterone on the lower urinary tract (bladder and urethra) have not been investigated to any great extent. The aim of this study was to determine whether androgen deprivation alters lower urinary tract metabolism. METHODS: A total of 16 rabbits were divided into four groups of four rabbits each. Each rabbit in groups 1-3 underwent surgical bilateral castration for duration of 1, 2 , and 4 weeks, and group 4 underwent sham operations. Sections of bladder body and base wall and mucosa, urethra and corpora were isolated, frozen, and stored under liquid nitrogen. The activities of citrate synthase-thapsigargin sensitive Ca(2+) ATPase (Sarco/Endoplasmic Reticulum Ca(2+ )ATPase [SERCA]), and choline acetyl-transferase were examined as markers for mitochondrial function, sarcoplasmic reticular calcium storage and release, and cholinergic nerve function, respectively. RESULTS: The activity of SR function indicator, Ca(2+) ATPase was significantly higher in the control corpora than in the control bladder or urethra. Castration resulted in decreased activity in the mitochondria specific enzyme, citrate synthase, the activity of which was greatest in the urethra and lowest in the corpora. Cholinergic nerve density indicator, choline acetyl-transferase activity was greatest in the bladder body and lowest in the urethra. CONCLUSIONS: Our data indicate that (1) significant differences exist in the activities of all three enzymes in the various organs associated with the lower urinary tract; and (2) that castration results in significant alterations in the activities of all three enzymes in the bladder body, base, urethra, and corpora.

Effect of castration on male rabbit lower urinary tract tissue enzymes. Publishing Authors By Initials

YS Juan,B Onal,S Broadaway,J Cosgrove,RE Leggett,C Whitbeck,E De,R Sokol,RM Levin,

For similar urogenital system: urinary tract research abstracts see: urogenital system: urinary tract research

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MEDLINE DATE:

Effect of castration on male rabbit lower urinary tract tissue enzymes. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular and cellular biochemistry

VOLUME: 301

Page Numbers: 227-33

Journal Abbreviation: Mol. Cell. Biochem.

ISSN: 0300-8177

DAY: 9

MONTH: 02

YEAR: 2007

Effect of castration on male rabbit lower urinary tract tissue enzymes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 364456

Effect of castration on male rabbit lower urinary tract tissue enzymes. Keywords Mesh Terms:

KEYWORDS: Urinary Tract

MESH TERMS: enzymology

Chemical & Substance for Abstract: Effect of castration on male rabbit lower urinary tract tissue enzymes. Information

Substance Name: Calcium-Transporting ATPases

Registry Number: EC 3.6.1.8

Grant and Affiliation Information for Effect of castration on male rabbit lower urinary tract tissue enzymes.

AFFILIATION: Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan.

Country: Netherlands

AGENCY: United States NIDDK

GRANT: R01 DK067114

ACRONYM: DK

MEDLINETA: Mol Cell Biochem

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