Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro.

Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro. Research Abstract Details 

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Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro. Abstract Text:

J Kipnes,A L Carlberg,G A Loredo,J Lawler,R S Tuan,D J Hall,

OBJECTIVE: Cartilage oligomeric matrix protein (COMP) mutations have been identified as responsible for two arthritic disorders, multiple epiphyseal dysplasia (MED) and pseudoachondroplasia (PSACH). However, the function of COMP in chondrogenic differentiation is largely unknown. Our investigation focuses on analyzing the function of normal COMP protein in cartilage biology. METHODS AND RESULTS: To explore the function of COMP we make use of an in vitro model system for chondrogenesis, consisting of murine C3H10T1/2 mesenchymal cells maintained as a high-density micromass culture and stimulated with bone morphogenetic protein 2 (BMP-2). Under these culture conditions, C3H10T1/2 cells undergo active chondrogenesis in a manner analogous to that of embryonic limb mesenchymal cells, and have been shown to serve as a valid model system to investigate the mechanisms regulating mesenchymal chondrogenesis. Our results indicate that ectopic COMP expression enhances several early aspects of chondrogenesis induced by BMP-2 in this system, indicating that COMP functions in part to positively regulate chondrogenesis. Additionally, COMP has inhibitory effects on proliferation of cells in monolayer. However, at later times in micromass culture, ectopic COMP expression in the presence of BMP-2 causes an increase in apoptosis, with an accompanying reduction in cell numbers in the micromass culture. However, the remaining cells retain their chondrogenic phenotype. CONCLUSIONS: These data suggest that COMP and BMP-2 signaling converge to regulate the fate of these cells in vitro by affecting both early and late stages of chondrogenesis.

Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro. Publishing Authors By Initials

J Kipnes,AL Carlberg,GA Loredo,J Lawler,RS Tuan,DJ Hall,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Osteoarthritis and cartilage / OARS, Osteoarthriti

VOLUME: 11

Page Numbers: 442-54

Journal Abbreviation: Osteoarthr. Cartil.

ISSN: 1063-4584

DAY: 19

MONTH: Jun

YEAR: 2003

Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9305697

Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: methods

Chemical & Substance for Abstract: Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro.

AFFILIATION: Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH, MSC 5755 Bldg 13, Rm 3W17, Bethesda, MD 20892, USA.

Country: England

AGENCY: United States NHLBI

GRANT: HL49081

ACRONYM: HL

MEDLINETA: Osteoarthritis Cartilage

REFSOURCE: Osteoarthritis Cartilage. 2003 Nov;11(11

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