Effect of calcium on the endogenous phosphorylation of mouse brain microsomes in vitro.

Effect of calcium on the endogenous phosphorylation of mouse brain microsomes in vitro. Research Abstract Details 

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Effect of calcium on the endogenous phosphorylation of mouse brain microsomes in vitro. Abstract Text:

D Satomi,

1. The endogenous phosphorylation of mouse brain microsomes was studied using the technique of acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). 2. It was found that specific proteins and lipids in brain microsomes were phosphorylated by the terminal phosphate of ATP under appropriate conditions. Six peaks of radioactivity were observed on SDS-polyacrylamide gel electrophoresis of 32Pi-labelled brain microsomes. The peaks were designated as P-I, P-II, P-III, P-IV, P-V, and P-VI. The peaks from P-I to P-V, which consist of phosphoproteins, underwent rapid dephosphorylation. On the other hand, P-VI, which consists of phospholipids, remained unaffected even after the complete hydrolysis of added ATP. 3. With the addition of 100 muM CaCl2 to the assay medium, the phosphorylation of brain microsomal proteins was stimulated; in the regions of P-I, P-II, and P-III, the amounts of 32Pi incorporation were approximately twice the 32Pi incorporation in the absence of Ca2+. On the other hand, 32Pi incorporation into P-VI was unaffected irrespective of the presence or absence of 100 muM CaCl2. In the presence of higher concentrations of Ca2+ (1-10 mM), the phosphorylation of all components was inhibited.

Effect of calcium on the endogenous phosphorylation of mouse brain microsomes in vitro. Publishing Authors By Initials

D Satomi,

For similar proteins: nerve tissue proteins research abstracts see: proteins: nerve tissue proteins research

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Effect of calcium on the endogenous phosphorylation of mouse brain microsomes in vitro. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 82

Page Numbers: 1695-700

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1977

Effect of calcium on the endogenous phosphorylation of mouse brain microsomes in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Effect of calcium on the endogenous phosphorylation of mouse brain microsomes in vitro. Keywords Mesh Terms:

KEYWORDS: Nerve Tissue Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Effect of calcium on the endogenous phosphorylation of mouse brain microsomes in vitro. Information

Substance Name: Calcium

Registry Number: 7440-70-2

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Country: JAPAN

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MEDLINETA: J Biochem

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