Effect of arsenic trioxide on uveal melanoma cell proliferation in vitro.

Effect of arsenic trioxide on uveal melanoma cell proliferation in vitro. Research Abstract Details 

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Effect of arsenic trioxide on uveal melanoma cell proliferation in vitro. Abstract Text:

Chunfang Wang,Bin Li,Haiyan Zhang,Weihong Li,Jost B Jonas,Chunfang Wang,Bin Li,Haiyan Zhang,Weihong Li,Jost B Jonas,Chunfang Wang,Bin Li,Haiyan Zhang,Weihong Li,Jost B Jonas,Chunfang Wang,Bin Li,Haiyan Zhang,Guangpu Shi,Weihong Li,Jost B Jonas,

AIMS: The aim of this study was to investigate the effect of arsenic trioxide (ATO) on the growth of cultured uveal melanoma cells (OCM-1 cell line). METHODS: Cultured OCM-1 cells were treated with 0.75-24 microM of ATO for 4-96 h. Cell viability was evaluated with the methylthiazoletetrazolium (MTT) assay as proliferation test. Apoptotic and necrotic cells were quantified using flow cytometry following Annexin-V/PI double stain. The cell morphology alteration was examined by light and electron transmission microscopy. To investigate the underlying mechanism of ATO-induced apoptosis and necrosis, glutathione peroxidase activity was measured, and mitochondrial membrane potentials were quantified using confocal microscopy. RESULTS: In the MTT assays, OCM-1 cell growth was inhibited at ATO concentrations of 1.5-24 microM. The 50% inhibitory concentration of ATO was 16.8 microM. At ATO concentrations of 12 and 24 microM, apoptosis and necrosis were induced after 24 h of incubation as shown by light and transmission electron microscopy. At ATO concentrations of 12 and 24 microM, the glutathione peroxidase activity was significantly (p < 0.05) reduced after 24 h of incubation, and the mitochondrial membrane potentials were significantly (p < 0.01) decreased after 4, 12 and 24 h of incubation. CONCLUSION: ATO inhibited ocular melanoma cell line growth at concentrations of 1.5-24 microM of ATO in a dose- and time-dependent manner by inducing apoptosis and necrosis of the tumor cells. The concurringly decreased glutathione peroxidase activity and the reduced mitochondrial membrane potentials may be possible underlying mechanisms for the apoptosis and necrosis of the tumor cells.

Effect of arsenic trioxide on uveal melanoma cell proliferation in vitro. Publishing Authors By Initials

C Wang,B Li,H Zhang,W Li,JB Jonas,C Wang,B Li,H Zhang,W Li,JB Jonas,C Wang,B Li,H Zhang,W Li,JB Jonas,C Wang,B Li,H Zhang,G Shi,W Li,JB Jonas,

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Effect of arsenic trioxide on uveal melanoma cell proliferation in vitro. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Ophthalmic research

VOLUME: 39

Page Numbers: 302-7

Journal Abbreviation: Ophthalmic Res.

ISSN: 1423-0259

DAY: 22

MONTH: 10

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 267442

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Grant and Affiliation Information for Effect of arsenic trioxide on uveal melanoma cell proliferation in vitro.

AFFILIATION: Beijing Institute of Ophthalmology, Beijing TongRen Eye Center, Beijing TongRen Hospital, Beijing, China.

Country: Switzerland

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MEDLINETA: Ophthalmic Res

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