Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts.

Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts. Research Abstract Details 

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Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts. Abstract Text:

K Uemura,E Sugiyama,C Tamai,A Hara,T Taketomi,N S Radin,

1-Phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), an effective inhibitor of UDP-glucose:ceramide glucosyltransferase, caused growth inhibition of cultured rabbit skin fibroblasts in a dose-dependent manner. At 50 microM both threo and erythro isomers of PDMP completely suppressed the cell growth. Major gangliosides of the fibroblasts, GM3 and GD3, were greatly reduced in amounts in the presence of threo-PDMP and accumulation of ceramides was observed. Surface labeling with galactose oxidase and [3H]NaBH4 demonstrated that neural glycosphingolipids with four or more sugars present on the surface of control cells were not detectable when the fibroblasts were grown in medium containing threo-PDMP. Metabolic labeling of cellular glycosphingolipids with [14C]-galactose showed reduced incorporation of radioactivity into gangliosides and neutral glycosphingolipids when threo-PDMP was present in the medium. In contrast, the erythro isomer of PDMP did not affect the biosynthesis of glycosphingolipids, a result suggesting that the inhibitory effect of erythro-PDMP on cell growth was due to a mechanism other than the inhibition of glucosyltransferase.

Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts. Publishing Authors By Initials

K Uemura,E Sugiyama,C Tamai,A Hara,T Taketomi,NS Radin,

For similar integumentary system: skin research abstracts see: integumentary system: skin research

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Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biochemistry

VOLUME: 108

Page Numbers: 525-30

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1990

Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts. Keywords Mesh Terms:

KEYWORDS: Skin

MESH TERMS: cytology

Chemical & Substance for Abstract: Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts. Information

Substance Name: Glucosyltransferases

Registry Number: EC 2.4.1.-

Grant and Affiliation Information for Effect of an inhibitor of glucosylceramide synthesis on cultured rabbit skin fibroblasts.

AFFILIATION: Department of Lipid Biochemistry, Shinshu University School of Medicine, Matsumoto.

Country: JAPAN

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MEDLINETA: J Biochem

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