[Effect of adenoviral N-methylpurine DNA glycosylase overexpression on chemosensitivity of human osteosarcoma cells]

[Effect of adenoviral N-methylpurine DNA glycosylase overexpression on chemosensitivity of human osteosarcoma cells] Research Abstract Details 

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[Effect of adenoviral N-methylpurine DNA glycosylase overexpression on chemosensitivity of human osteosarcoma cells] Abstract Text:

Dong Wang,Zhao-yang Zhong,Qin-hong Zhang,Zeng-peng Li,Mark R Kelley,

OBJECTIVE: The overexpression of N-methylpurine DNA glycosylase (MPG) may imbalance the DNA base excision repair (BER) to sensitize tumor cells to current DNA damage chemotherapy. In an effort to improve the efficacy of cancer chemotherapy, we have constructed adenoviral vector of MPG, to study its ability to sensitize human osteosarcoma cell HOS to DNA damage agents. METHODS: The adenoviral infection and MPG expression, as well as enzyme activity were determined by flow cytometry, Western blot, and HEX labeled oligonucleotide-based assay respectively. The cell survival/proliferation was measured using MTS, SRB, and [(3)H] thymidine incorporation assay. Apoptosis cell death was assayed by flow cytometry after treatment using phycoerythin (PE)-conjugated Annexin V and 7-amino-actinomycin (7-AAD). RESULTS: A 10 MOI of recombinant nonreplicating adenovirus was found to infect more than 90% of HOS cells within 24 hours by EGFP fluorescence, in which the MPG overexpression and MPG enzyme activity were also detected. The MPG overexpression HOS cells were significantly more sensitive to the DNA damage agents, including MMS, MNNG, and TMZ, with changes in the IC50 of 6.0, 4.5, and 2.5 fold respectively. CONCLUSIONS: These data establish transient MPG overexpression as a potential therapeutic approach for increasing HOS cellular sensitivity to DNA damage agent chemotherapy.

[Effect of adenoviral N-methylpurine DNA glycosylase overexpression on chemosensitivity of human osteosarcoma cells] Publishing Authors By Initials

D Wang,ZY Zhong,QH Zhang,ZP Li,MR Kelley,

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[Effect of adenoviral N-methylpurine DNA glycosylase overexpression on chemosensitivity of human osteosarcoma cells] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua bing li xue za zhi Chinese journal of pat

VOLUME: 35

Page Numbers: 352-6

Journal Abbreviation: Zhonghua Bing Li Xue Za Zhi

ISSN: 0529-5807

DAY: 12

MONTH: Jun

YEAR: 2006

[Effect of adenoviral N-methylpurine DNA glycosylase overexpression on chemosensitivity of human osteosarcoma cells] Information

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LANGUAGE: chi

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AFFILIATION: Cancer Center and Department of Pathology, Daping Hospital, Third Military Medical University, Chongqing 400042, China. dongwang64@hotmail.com

Country: China

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MEDLINETA: Zhonghua Bing Li Xue Za Zhi

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