Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel.

Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. Research Abstract Details 

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Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. Abstract Text:

Shane N White,Michael L Paine,Amanda Y W Ngan,Vetea G Miklus,Wen Luo,HongJun Wang,Malcolm L Snead,

Dentin sialophosphpoprotein (Dspp) is transiently expressed in the early stage of secretory ameloblasts. The secretion of ameloblast-derived Dspp is short-lived, correlates to the establishment of the dentinoenamel junction (DEJ), and is consistent with Dspp having a role in producing the specialized first-formed harder enamel adjacent to the DEJ. Crack diffusion by branching and dissipation within this specialized first-formed enamel close to the DEJ prevents catastrophic interfacial damage and tooth failure. Once Dspp is secreted, it is subjected to proteolytic cleavage that results in two distinct proteins referred to as dentin sialoprotein (Dsp) and dentin phosphoprotein (Dpp). The purpose of this study was to investigate the biological and mechanical contribution of Dsp and Dpp to enamel formation. Transgenic mice were engineered to overexpress either Dsp or Dpp in their enamel organs. The mechanical properties (hardness and toughness) of the mature enamel of transgenic mice were compared with genetically matched and age-matched nontransgenic animals. Dsp and Dpp contributions to enamel formation greatly differed. The inclusion of Dsp in bulk enamel significantly and uniformly increased enamel hardness (20%), whereas the inclusion of Dpp weakened the bulk enamel. Thus, Dsp appears to make a unique contribution to the physical properties of the DEJ. Dsp transgenic animals have been engineered with superior enamel mechanical properties.

Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. Publishing Authors By Initials

SN White,ML Paine,AY Ngan,VG Miklus,W Luo,H Wang,ML Snead,

For similar genetic processes: gene expression regulation: protein modification, translational: protein processing, post-translational research abstracts see: genetic processes: gene expression regulation: protein modification, translational: protein processing, post-translational research

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Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 5340-5

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 22

MONTH: 12

YEAR: 2006

Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. Keywords Mesh Terms:

KEYWORDS: Protein Processing, Post-Translational

MESH TERMS: physiology

Chemical & Substance for Abstract: Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel. Information

Substance Name: Protein Precursors

Registry Number: 0

Grant and Affiliation Information for Ectopic expression of dentin sialoprotein during amelogenesis hardens bulk enamel.

AFFILIATION: School of Dentistry, University of California at Los Angeles, Los Angeles, California 90095, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: DE014189

ACRONYM: DE

MEDLINETA: J Biol Chem

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