Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate.

Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate. Research Abstract Details 

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Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate. Abstract Text:

J W Vehof,J Mahmood,H Takita,M A van't Hof,Y Kuboki,P H Spauwen,J A Jansen,

The osteoinductive properties of porous titanium fiber mesh, with or without a calcium phosphate coating and loaded with recombinant human bone morphogenic protein-2 (rhBMP-2) or rhBMP-2 and native bovine BMP (S-300) were investigated in a rat ectopic assay model. A total of 112 calcium phosphate-coated and 112 noncoated porous titanium implants, either loaded with rhBMP-2 and S-300 or loaded with rhBMP-2 alone, were subcutaneously placed in 56 Wistar-King rats. The rats were killed 5, 10, 20, and 40 days postoperatively, and the implants were retrieved.Histologic analysis demonstrated that all growth factor and carrier combinations induced ectopic cartilage and bone formation at 5 and 10 days, respectively. At 20 days, bone formation increased and was characterized by trabecular bone and bone marrow-like tissue. At 40 days, more lamellar bone and hemopoietic bone marrow-like tissue were present. At both times, more bone had been formed in calcium phosphate-coated implants than in noncoated samples. Further, in rhBMP-2 and S-300-loaded specimens, bone formation was higher than in rhBMP-2 only-loaded specimens. In rhBMP-2 only-loaded specimens, bone formation was mainly localized inside the mesh material, whereas in specimens loaded with both rhBMP-2 and S-300, the bone was localized inside and surrounding the titanium mesh. The histological findings were confirmed by calcium content and alkaline phosphatase activity measurements. In addition, all specimens showed osteocalcin expression as early as 5 days postoperatively.Our results show that the combination of titanium mesh with BMPs can induce ectopic bone formation and that this bone formation seems to be similar to "enchondral" ossification. In addition, a thin calcium phosphate coating can have a beneficial effect on the bone-inducing properties of a scaffold material. Finally, rhBMP-2 and native BMP act synergistically in ectopic bone induction.

Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate. Publishing Authors By Initials

JW Vehof,J Mahmood,H Takita,MA van't Hof,Y Kuboki,PH Spauwen,JA Jansen,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Plastic and reconstructive surgery

VOLUME: 108

Page Numbers: 434-43

Journal Abbreviation: Plast. Reconstr. Surg.

ISSN: 0032-1052

DAY: 19

MONTH: Aug

YEAR: 2001

Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate. Information

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LANGUAGE: eng

NlmUniqueID: 1306050

Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Ectopic bone formation in titanium mesh loaded with bone morphogenetic protein and coated with calcium phosphate.

AFFILIATION: Departments of Biomaterials and Preventive and Restorative Dentistry, College of Dental Science, University Medical Center Nijmegen, The Netherlands.

Country: United States

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MEDLINETA: Plast Reconstr Surg

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