Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria.

Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria. Research Abstract Details 

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Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria. Abstract Text:

Anita Willmann,Michael Weiss,Uwe Nehls,

A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was identified in an EST project of functional Amanita muscaria/poplar ectomycorrhizas. Heterologous expression of the entire AmAMT2 coding region in yeast revealed the corresponding protein to be a high-affinity ammonium importer. In axenically grown Amanita hyphae AmAMT2 expression was strongly repressed by nitrogen, independent of whether the offered nitrogen source was transported by AmAMT2 or not. In functional ectomycorrhizas the AmAMT2 transcript level was further decreased in both hyphal networks (sheath and Hartig net), while extraradical hyphae revealed strong gene expression. Together our data suggest that (1) AmAMT2 expression is regulated by the endogenous nitrogen content of hyphae and (2) fungal hyphae in ectomycorrhizas are well supported with nitrogen even when the extraradical mycelium is nitrogen limited. As a consequence of AmAMT2 repression in mycorrhizas, ammonium can be suggested as a potential nitrogen source delivered by fungal hyphae in symbiosis.

Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria. Publishing Authors By Initials

A Willmann,M Weiss,U Nehls,

For similar genetic processes: mutagenesis: suppression, genetic research abstracts see: genetic processes: mutagenesis: suppression, genetic research

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Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Current genetics

VOLUME: 51

Page Numbers: 71-8

Journal Abbreviation: Curr. Genet.

ISSN: 0172-8083

DAY: 28

MONTH: 10

YEAR: 2006

Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria. Information

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LANGUAGE: eng

NlmUniqueID: 8004904

Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria. Keywords Mesh Terms:

KEYWORDS: Suppression, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria. Information

Substance Name: ammonium transporters, plant

Registry Number: 0

Grant and Affiliation Information for Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria.

AFFILIATION: Physiologische Okologie der Pflanzen, Eberhard Karls Universität, Auf der Morgenstelle 1, 72076, Tübingen, Germany.

Country: United States

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MEDLINETA: Curr Genet

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