Early morphological events of in vitro fertilized bovine oocytes with frozen-thawed spermatozoa.

Early morphological events of in vitro fertilized bovine oocytes with frozen-thawed spermatozoa. Research Abstract Details 

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Early morphological events of in vitro fertilized bovine oocytes with frozen-thawed spermatozoa. Abstract Text:

K Saeki,H Kato,Y Hosoi,M Miyake,K Utsumi,A Iritani,

Bovine follicular oocytes were matured and inseminated in vitro with spermatozoa capacitated in vitro. The first evidence of sperm penetration was observed at 3 h after insemination. The penetration rate increased until 5 h, and reached a maximum rate (92%) at 5 h. Decondensation of the sperm head and pronuclear formation were observed 4 h and 7 h after insemination, respectively. Female chromatins of all penetrated oocytes were activated at 3 h, and female pronuclei were formed at 7 h after insemination. Percentages of oocytes with male and female pronuclei at 9 h were 88 and 94%. Polyspermy (4, 7, 19 and 29% at 4, 5, 7 and 9 h after insemination, respectively) and abnormal development of male pronuclei (6 and 7% at 7 and 9 h after insemination, respectively) were also seen.

Early morphological events of in vitro fertilized bovine oocytes with frozen-thawed spermatozoa. Publishing Authors By Initials

K Saeki,H Kato,Y Hosoi,M Miyake,K Utsumi,A Iritani,

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Early morphological events of in vitro fertilized bovine oocytes with frozen-thawed spermatozoa. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 35

Page Numbers: 1051-8

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: May

YEAR: 1991

Early morphological events of in vitro fertilized bovine oocytes with frozen-thawed spermatozoa. Information

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LANGUAGE: eng

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AFFILIATION: Department of Animal Science, College of Agriculture Kyoto University, Kyoto, 606 Japan.

Country: United States

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MEDLINETA: Theriogenology

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