Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae).

Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae). Research Abstract Details 

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Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae). Abstract Text:

Kim Hor Hee,Chiang Shiong Loh,Hock Hin Yeoh,Kim Hor Hee,Chiang Shiong Loh,Hock Hin Yeoh,

Plantlets of Dendrobium Chao Praya Smile maintained in vitro were induced to flower, which produced viable seeds within about 11 months. A two-layer (Gelrite-solidified layer topped with a layer of liquid medium of the same volume and composition) culture system containing benzyladenine (BA) at 11.1 muM induced the highest percent of flowering (45%) in plantlets within 6 months from germination. The percentage of inflorescence induction was increased to 72% by pre-selecting morphologically normal seedlings prior to two-layer culture. Plantlets in culture produced both complete (developmentally normal but smaller than flowers of field grown plants) and incomplete flowers. Pollen and female reproductive organs of in vitro-developed complete flowers were morphologically and anatomically similar to flowers of field grown plants. In addition, 65% of the pollen grains derived from in vitro-developed flower were tetrad suggesting that regular meiosis occurred during microsporogenesis. The percentage of germination of pollen grains derived from in vitro-developed flowers and flowers of field grown plants, incubated on modified Knops' medium for 8 days, were 18.2 and 52.8%, respectively. Despite a lower percentage of germination of the pollen grains derived from in vitro-developed flowers, flowers induced in culture could be self-pollinated and developed seedpods with viable seeds. Nearly 90% of these seeds developed into protocorms on germination in vitro. These seedlings were grown in culture and induced to flower in vitro again using the same procedure.

Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae). Publishing Authors By Initials

KH Hee,CS Loh,HH Yeoh,KH Hee,CS Loh,HH Yeoh,

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Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Plant cell reports

VOLUME: 26

Page Numbers: 2055-62

Journal Abbreviation: Plant Cell Rep.

ISSN: 0721-7714

DAY: 7

MONTH: 08

YEAR: 2007

Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae). Information

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LANGUAGE: eng

NlmUniqueID: 9880970

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Grant and Affiliation Information for Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae).

AFFILIATION: Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore, Singapore, 117543, dbslohcs@nus.edu.sg.

Country: Germany

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MEDLINETA: Plant Cell Rep

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