Early events of polyoma infection: adsorption, penetration and nuclear transport.

Early events of polyoma infection: adsorption, penetration and nuclear transport. Research Abstract Details 

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Early events of polyoma infection: adsorption, penetration and nuclear transport. Abstract Text:

Polyoma virions have different attachment proteins which are responsible for hemagglutination of erythrocytes and attachment to cultured mouse kidney cells (MKC). Virion binding studies demonstrated that MKC possess specific (productive infection) and nonspecific (nonproductive) receptors. Empty polyoma capsids have hemagglutination activity and bind to non-specific MKC receptors, but they are not capable of competing for specific virion cell receptors or preventing productive infection. Isoelectric focusing of the virion major capsid protein, VP1, separated this protein into six species (A through F). These species had identical amino acid sequences, but differed in degree of modification (phosphorylation, acetylation, sulfation and hydroxylation). Evidence based upon precipitation with specific antisera supports the view that VP1 species E is required for specific adsorption and that D and F are required for hemagglutination. The virion attachment domain has been localized to an 18 kilodalton fragment of the C-terminal region of VP1. Monopinocytotic vesicles containing 125I-labeled polyoma virions were isolated from infected MKC. A crosslinker was used to bind the MKC cell receptor(s) covalently to VP1 attachment protein, and a new 120 kilodalton band was identified by SDS-PAGE. An anti-idiotype antibody prepared against a neutralizing polyoma monoclonal antiody was used to identify a putative 50 kilodalton receptor protein from a detergent extract of MKC, as well as from MKC membrane preparation.

Early events of polyoma infection: adsorption, penetration and nuclear transport. Publishing Authors By Initials

For similar virus diseases: tumor virus infections research abstracts see: virus diseases: tumor virus infections research

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Early events of polyoma infection: adsorption, penetration and nuclear transport. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Transactions of the Kansas Academy of Science. Kan

VOLUME: 95

Page Numbers: 62-9

Journal Abbreviation: Trans. Kans. Acad. Sci.

ISSN: 0022-8443

DAY: 26

MONTH: Apr

YEAR: 1992

Early events of polyoma infection: adsorption, penetration and nuclear transport. Information

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LANGUAGE: eng

NlmUniqueID: 7506117

Early events of polyoma infection: adsorption, penetration and nuclear transport. Keywords Mesh Terms:

KEYWORDS: Tumor Virus Infections

MESH TERMS: physiopathology

Chemical & Substance for Abstract: Early events of polyoma infection: adsorption, penetration and nuclear transport. Information

Substance Name: VP1 protein, polyomavirus

Registry Number: 0

Grant and Affiliation Information for Early events of polyoma infection: adsorption, penetration and nuclear transport.

AFFILIATION: KS St U, Manhattan

Country: UNITED STATES

AGENCY: United States NCI

GRANT: CA07319

ACRONYM: CA

MEDLINETA: Trans Kans Acad Sci

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