Early events of Bacillus anthracis germination identified by time-course quantitative proteomics.

Early events of Bacillus anthracis germination identified by time-course quantitative proteomics. Research Abstract Details 

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Early events of Bacillus anthracis germination identified by time-course quantitative proteomics. Abstract Text:

Pratik Jagtap,George Michailidis,Ryszard Zielke,Angela K Walker,Nishi Patel,John R Strahler,Adam Driks,Philip C Andrews,Janine R Maddock,

Germination of Bacillus anthracis spores involves rehydration of the spore interior and rapid degradation of several of the protective layers, including the spore coat. Here, we examine the temporal changes that occur during B. anthracis spore germination using an isobaric tagging system. Over the course of 17 min from the onset of germination, the levels of at least 19 spore proteins significantly decrease. Included are acid-soluble proteins, several known and predicted coat proteins, and proteins of unknown function. Over half of these proteins are small (less than 100 amino acids) and would have been undetectable by conventional gel-based analysis. We also identified 20 proteins, whose levels modestly increased at the later time points when metabolism has likely resumed. Taken together, our data show that isobaric labeling of complex mixtures is particularly effective for temporal studies. Furthermore, we describe a rigorous statistical approach to define relevant changes that takes into account the nature of data obtained from multidimensional protein identification technology coupled with the use of isobaric tags. This study provides an expanded list of the proteins that may be involved in germination of the B. anthracis spore and their relative levels during germination.

Early events of Bacillus anthracis germination identified by time-course quantitative proteomics. Publishing Authors By Initials

P Jagtap,G Michailidis,R Zielke,AK Walker,N Patel,JR Strahler,A Driks,PC Andrews,JR Maddock,

For similar cells: spores: spores, bacterial research abstracts see: cells: spores: spores, bacterial research

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Early events of Bacillus anthracis germination identified by time-course quantitative proteomics. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proteomics

VOLUME: 6

Page Numbers: 5199-211

Journal Abbreviation: Proteomics

ISSN: 1615-9853

DAY: 3

MONTH: Oct

YEAR: 2006

Early events of Bacillus anthracis germination identified by time-course quantitative proteomics. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101092707

Early events of Bacillus anthracis germination identified by time-course quantitative proteomics. Keywords Mesh Terms:

KEYWORDS: Spores, Bacterial

MESH TERMS: physiology

Chemical & Substance for Abstract: Early events of Bacillus anthracis germination identified by time-course quantitative proteomics. Information

Substance Name: spore-specific proteins, Bacillus

Registry Number: 0

Grant and Affiliation Information for Early events of Bacillus anthracis germination identified by time-course quantitative proteomics.

AFFILIATION: Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109-1048, USA.

Country: Germany

AGENCY: United States PHS

GRANT: P41-18627

ACRONYM: GM

MEDLINETA: Proteomics

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