Early detection of typhoid by polymerase chain reaction.

Early detection of typhoid by polymerase chain reaction. Research Abstract Details 

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Early detection of typhoid by polymerase chain reaction. Abstract Text:

A Haque,J Ahmed,J A Qureshi,

Background: Typhoid is a common problem in developing countries. Cultivation of bacteria and serology (especially Widal test) give unacceptable levels of false-negative and false-positive results, respectively. Patients and Methods: In this study, a recently introduced polymerase chain reaction-based technique (which has 100% specificity for Salmonella typhi) was compared with blood culture and Widal test during the first week of illness of 82 suspected cases of typhoid. Results: The respective figures of positivity for PCR, blood culture and Widal test were 71.95%, 34.1%, and 36.5%. A control group of 20 healthy persons gave figures of 0%, 0%, and 33.3%, respectively. Conclusion: We conclude that this PCR-based technique is not only absolutely specific, but also very sensitive and, therefore, much superior to blood culture and Widal test for the early diagnosis of typhoid.

Early detection of typhoid by polymerase chain reaction. Publishing Authors By Initials

A Haque,J Ahmed,JA Qureshi,

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PUBMED ID PMID:

MEDLINE DATE: 1999 Jul-Aug

Early detection of typhoid by polymerase chain reaction. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Annals of Saudi medicine

VOLUME: 19

Page Numbers: 337-40

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ISSN: 0256-4947

DAY: 5

MONTH: 02

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 8507355

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Grant and Affiliation Information for Early detection of typhoid by polymerase chain reaction.

AFFILIATION: National Institute for Biotechnology and Genetic Engineering (NIBGE), and Department of Pathology, Punjab Medical College, Faisalabad, Pakistan.

Country: Saudi Arabia

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MEDLINETA: Ann Saudi Med

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