Dynamics of transcriptional and translational processes in hepatocytes cultured in a collagen sandwich.

Dynamics of transcriptional and translational processes in hepatocytes cultured in a collagen sandwich. Research Abstract Details 

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Dynamics of transcriptional and translational processes in hepatocytes cultured in a collagen sandwich. Abstract Text:

A mathematical model which simulates the dynamic behavior of the hepatocytes cultured in a collagen sandwich is presented. Using several independently determined experimental parameters (e.g., albumin gene nuclear runoff activity, the level of albumin mRNA, and the albumin secretion rate), we have used this model to calculate the in vivo albumin gene transcriptional rate (0.27 molecules per second per hepatocyte), the half-life of albumin mRNA (3.3 days) in cultured hepatocytes, and the albumin polypeptide elongation rate (10 amino acids per second). In addition, the characteristic time constants for the transient increases in transcription (5 days) and in translation (10 days) were also obtained. These bestfit parameters were used to predict the rate of albumin secretion in rescued hepatocytes. (c) 1993 John Wiley & Sons, Inc.

Dynamics of transcriptional and translational processes in hepatocytes cultured in a collagen sandwich. Publishing Authors By Initials

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Dynamics of transcriptional and translational processes in hepatocytes cultured in a collagen sandwich. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology and bioengineering

VOLUME: 41

Page Numbers: 593-8

Journal Abbreviation: Biotechnol. Bioeng.

ISSN: 0006-3592

DAY: 5

MONTH: Mar

YEAR: 1993

Dynamics of transcriptional and translational processes in hepatocytes cultured in a collagen sandwich. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Dynamics of transcriptional and translational processes in hepatocytes cultured in a collagen sandwich.

AFFILIATION: Surgical Services, Massachusetts General Hospital and the Shriners Burns Institute, Boston, Massachusetts 02114.

Country: United States

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MEDLINETA: Biotechnol Bioeng

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