Dynamics of increased neutrophil adhesion to ICAM-1 after contacting immobilized IL-8.

Dynamics of increased neutrophil adhesion to ICAM-1 after contacting immobilized IL-8. Research Abstract Details 

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Dynamics of increased neutrophil adhesion to ICAM-1 after contacting immobilized IL-8. Abstract Text:

Elena B Lomakina,Richard E Waugh,

Changing affinity of beta(2)-integrins on neutrophils for their ligands on endothelium is a critical, regulated step in the inflammatory response. In this report, the dynamics of the neutrophil response to the inflammatory chemokine interleukin-8 (IL-8) is examined. Human IL-8 was immobilized on beads and brought into contact with neutrophils selected from whole blood samples. Resulting changes in cellular adhesion were assessed by measuring the adhesion frequency between a human neutrophil and a bead coated with the endothelial ligand ICAM-1 (intercellular adhesion molecule-1). Cells engulfed the IL-8 coated beads within a few tens of seconds, and most of the cells exhibited an increase in adhesion to ICAM-1 after approximately 5 to 10 min of contact with IL-8 at room temperature (3 to 5 min at 37 degrees C). Neither monocyte chemotactic protein-1 (MCP-1) nor anti-CD45-coated beads caused any changes in adhesion to ICAM-1. IL-8 induced adhesion was blocked by antibody against CD18. At lower surface density of chemokine, approximately 20% of IL-8 coated beads adhered but were not engulfed by the cells, although the increase in adhesion for ICAM-1 was still effected. Heterogeneity in the cellular response and variability between donors was also noted.

Dynamics of increased neutrophil adhesion to ICAM-1 after contacting immobilized IL-8. Publishing Authors By Initials

EB Lomakina,RE Waugh,

For similar natural sciences: chemistry: chemistry, physical: surface properties research abstracts see: natural sciences: chemistry: chemistry, physical: surface properties research

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Dynamics of increased neutrophil adhesion to ICAM-1 after contacting immobilized IL-8. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Annals of biomedical engineering

VOLUME: 34

Page Numbers: 1553-63

Journal Abbreviation:

ISSN: 0090-6964

DAY: 20

MONTH: 09

YEAR: 2006

Dynamics of increased neutrophil adhesion to ICAM-1 after contacting immobilized IL-8. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 361512

Dynamics of increased neutrophil adhesion to ICAM-1 after contacting immobilized IL-8. Keywords Mesh Terms:

KEYWORDS: Surface Properties

MESH TERMS: metabolism

Chemical & Substance for Abstract: Dynamics of increased neutrophil adhesion to ICAM-1 after contacting immobilized IL-8. Information

Substance Name: Intercellular Adhesion Molecule-1

Registry Number: 126547-89-5

Grant and Affiliation Information for Dynamics of increased neutrophil adhesion to ICAM-1 after contacting immobilized IL-8.

AFFILIATION: Department of Pharmacology and Physiology, University of Rochester, Medical Center, Rochester, 601 Elmwood Avenue, Box 711, NY 14642, USA. Elena_Lomakina@URMC.Rochester.edu

Country: United States

AGENCY: United States NHLBI

GRANT: P01 HL 18208

ACRONYM: HL

MEDLINETA: Ann Biomed Eng

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