Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern.

Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern. Research Abstract Details 

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Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern. Abstract Text:

Ann D Skillman,James J Nagler,Sharon E Hook,Jack A Small,Irvin R Schultz,

17alpha-Ethynylestradiol (EE2) is a synthetic estrogen identified in sewage effluents. To understand better the absorption kinetics of EE2 and the induction of vitellogenin (VTG) and estrogen receptor alpha (ERalpha) mRNA, we subjected male rainbow trout (Onchorynchus mykiss) to continuous water exposures of 125 ng/L of EE2 for up to 61 d. Trout were either repetitively sampled for blood plasma or serially killed at selected time intervals. Vitellogenin, ERalpha mRNA, and EE2 were measured using enzyme-linked immunosorbent assay and using quantitative polymerase chain reaction and gas chromatography-mass spectrometry, respectively. In separate experiments, trout were exposed to EE2 for 7 d, and hepatic gene expression was assessed using a low- and high-density cDNA microarray. The EE2 was rapidly absorbed by the trout, with an apparent equilibrium at 16 h in plasma and liver. The ERalpha mRNA levels also increased rapidly, reaching near-peak levels by 48 h. In contrast, plasma levels of VTG continuously increased for 19 d. After 61 d, tissues with the highest levels of VTG were the liver, kidney, and testes. Microarray-based gene expression studies provided unexpected results. In some cases, known estrogen-responsive genes (e.g., ERalpha) were unresponsive, whereas many of the genes that have no apparent link to estrogen function or EE2 toxicity were significantly altered in expression. Of the two microarray approaches tested in the present study, the high-density array appeared to be superior because of the improved quality of the hybridization signal and the robustness of the response in terms of the number of genes identified as being EE2 responsive.

Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern. Publishing Authors By Initials

AD Skillman,JJ Nagler,SE Hook,JA Small,IR Schultz,

For similar proteins: egg proteins: vitellins: vitellogenins research abstracts see: proteins: egg proteins: vitellins: vitellogenins research

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Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Environmental toxicology and chemistry / SETAC

VOLUME: 25

Page Numbers: 2997-3005

Journal Abbreviation: Environ. Toxicol. Chem.

ISSN: 0730-7268

DAY: 3

MONTH: Nov

YEAR: 2006

Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8308958

Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern. Keywords Mesh Terms:

KEYWORDS: Vitellogenins

MESH TERMS: blood

Chemical & Substance for Abstract: Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern. Information

Substance Name: Ethinyl Estradiol

Registry Number: 57-63-6

Grant and Affiliation Information for Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern.

AFFILIATION: Pacific Northwest National Laboratory-Marine Sciences Laboratory, 1529 West Sequim Bay Road, Sequim, Washington 98382, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: 5R01ES012446-03

ACRONYM: ES

MEDLINETA: Environ Toxicol Chem

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