Dynamics and heterogeneity of bovine hippocampal membranes: role of cholesterol and proteins.

Dynamics and heterogeneity of bovine hippocampal membranes: role of cholesterol and proteins. Research Abstract Details 

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Dynamics and heterogeneity of bovine hippocampal membranes: role of cholesterol and proteins. Abstract Text:

Soumi Mukherjee,Mamata Kombrabail,G Krishnamoorthy,Amitabha Chattopadhyay,

The structural and dynamic consequence of alterations in membrane lipid composition (specifically cholesterol) in neuronal membranes is poorly understood. Previous work from our laboratory has established bovine hippocampal membranes as a convenient natural source for studying neuronal receptors. In this paper, we have explored the role of cholesterol and proteins in the dynamics and heterogeneity of bovine hippocampal membranes using fluorescence lifetime distribution analysis of the environment-sensitive fluorescent probe Nile Red incorporated into such membranes by the maximum entropy method (MEM), and time-resolved fluorescence anisotropy measurements. The peak position and the width of the lifetime distribution of Nile Red show a progressive reduction with increasing cholesterol depletion from native hippocampal membranes indicating that the extent of heterogeneity decreases with decrease in membrane cholesterol content. This is accompanied by a concomitant decrease of the fluorescence anisotropy and rotational correlation time. Our results point out that the microenvironment experienced by Nile Red is relatively insensitive to the presence of proteins in hippocampal membranes. Interestingly, Nile Red lifetime distribution in liposomes of lipid extracts is similar to that of native membranes indicating that proteins do not contribute significantly to the high level of heterogeneity observed in native membranes. These results could be relevant in understanding the neuronal diseases characterized by defective membrane lipid metabolism.

Dynamics and heterogeneity of bovine hippocampal membranes: role of cholesterol and proteins. Publishing Authors By Initials

S Mukherjee,M Kombrabail,G Krishnamoorthy,A Chattopadhyay,

For similar natural sciences: chemistry: chemistry, physical: phase transition research abstracts see: natural sciences: chemistry: chemistry, physical: phase transition research

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Dynamics and heterogeneity of bovine hippocampal membranes: role of cholesterol and proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimica et biophysica acta

VOLUME: 1768

Page Numbers: 2130-44

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 2

MONTH: 06

YEAR: 2007

Dynamics and heterogeneity of bovine hippocampal membranes: role of cholesterol and proteins. Information

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LANGUAGE: eng

NlmUniqueID: 217513

Dynamics and heterogeneity of bovine hippocampal membranes: role of cholesterol and proteins. Keywords Mesh Terms:

KEYWORDS: Phase Transition

MESH TERMS: metabolism

Chemical & Substance for Abstract: Dynamics and heterogeneity of bovine hippocampal membranes: role of cholesterol and proteins. Information

Substance Name: Cholesterol

Registry Number: 57-88-5

Grant and Affiliation Information for Dynamics and heterogeneity of bovine hippocampal membranes: role of cholesterol and proteins.

AFFILIATION: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.

Country: Netherlands

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MEDLINETA: Biochim Biophys Acta

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