Dynamic changes of estradiol and progesterone concentrations during in vitro oocyte maturation in cattle.

Dynamic changes of estradiol and progesterone concentrations during in vitro oocyte maturation in cattle. Research Abstract Details 

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Dynamic changes of estradiol and progesterone concentrations during in vitro oocyte maturation in cattle. Abstract Text:

K P Xu,R ,T Greve,

Two culture techniques, an open system using 1.0-ml medium and a covered system using 50-mul droplets of medium covered by paraffin oil, used for in vitro bovine follicular oocyte maturation were compared. Estradiol-17beta (E2) was added to Ham's F-10 medium together with other supplementations. In the absence of oocytes, E2 concentration remained unchanged in the 1.0-ml open system, but it decreased gradually in the 50-mul covered system. In the presence of oocytes, E2 increased significantly in the 1.0-ml open system, but this increase could not be detected in the 50-mul covered system. Progesterone (P4) concentration increased in both systems, but it was much higher in the 1.0-ml culture than in the 50-mul culture. The two culture systems allowed an identical nuclear oocyte maturation rate of 88.6 vs 87.0%, a sperm penetration rate of 54.3 vs 59.6%, and a polyspermy rate of 6.8 vs 11.6% for 1.0-ml and 50-mul systems, respectively. The cleavage rate, however, differed significantly (78.3 vs 41.7% for 1.0-ml and 50-mul systems, respectively). It is concluded that diffusion of steroids into the paraffin oil occurs and may affect the cleavage rate but not the maturation or penetration rate.

Dynamic changes of estradiol and progesterone concentrations during in vitro oocyte maturation in cattle. Publishing Authors By Initials

KP Xu,R ,T Greve,

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Dynamic changes of estradiol and progesterone concentrations during in vitro oocyte maturation in cattle. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 30

Page Numbers: 245-55

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Aug

YEAR: 1988

Dynamic changes of estradiol and progesterone concentrations during in vitro oocyte maturation in cattle. Information

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LANGUAGE: eng

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AFFILIATION: Department of Animal Reproduction. Royal Veterinary and Agricultural University. Bülowsvej 13, DK-1870 Frederiksberg C, Denmark.

Country: United States

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MEDLINETA: Theriogenology

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