Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase.

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase. Abstract Text:

Michael D Hamilton,Anthony A Nuara,Don B Gammon,R Mark Buller,David H Evans,

Vaccinia virus DNA polymerase catalyzes duplex-by-duplex DNA joining reactions in vitro and many features of these recombination reactions are reprised in vivo. This can explain the intimate linkage between virus replication and genetic recombination. However, it is unclear why these apparently ordinary polymerases exhibit this unusual catalytic capacity. In this study, we have used different substrates to perform a detailed investigation of the mechanism of duplex-by-duplex recombination catalyzed by vaccinia DNA polymerase. When homologous, blunt-ended linear duplex substrates are incubated with vaccinia polymerase, in the presence of Mg2+ and dNTPs, the appearance of joint molecules is preceded by the exposure of complementary single-stranded sequences by the proofreading exonuclease. These intermediates anneal to form a population of joint molecules containing hybrid regions flanked by nicks, 1-5 nt gaps, and/or short overhangs. The products are relatively resistant to exonuclease (and polymerase) activity and thus accumulate in joining reactions. Surface plasmon resonance (SPR) measurements showed the enzyme has a relative binding affinity favoring blunt-ended duplexes over molecules bearing 3'-recessed gaps. Recombinant duplexes are the least favored ligands. These data suggest that a particular combination of otherwise ordinary enzymatic and DNA-binding properties, enable poxvirus DNA polymerases to promote duplex joining reactions.

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase. Publishing Authors By Initials

MD Hamilton,AA Nuara,DB Gammon,RM Buller,DH Evans,

For similar investigative techniques: chemistry, analytical: surface plasmon resonance research abstracts see: investigative techniques: chemistry, analytical: surface plasmon resonance research

PUBMED ID PMID:

MEDLINE DATE:

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nucleic acids research

VOLUME: 35

Page Numbers: 143-51

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 7

MONTH: 12

YEAR: 2006

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 411011

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase. Keywords Mesh Terms:

KEYWORDS: Surface Plasmon Resonance

MESH TERMS: metabolism

Chemical & Substance for Abstract: Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase. Information

Substance Name: DNA-Directed DNA Polymerase

Registry Number: EC 2.7.7.7

Grant and Affiliation Information for Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase.

AFFILIATION: Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, The University of Alberta, Edmonton, AB, Canada T6G 2H7.

Country: England

AGENCY: United States NIAID

GRANT: N01-AI-15436

ACRONYM: AI

MEDLINETA: Nucleic Acids Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase Related Publications

• A national needs assessment of nurses providing health care to adolescents.
• Application of pharmacological principles to benzodiazepine therapy.
• Biologic agents as adjunctive therapy for prostate cancer: a rationale for use with androgen deprivation.
• Black-white differences in health-related quality of life among older adults.
• Cannabinoids: the separation of central from peripheral effects on a structural basis.
• Chiropractic clinical research: progress and recommendations.
• Determinants of cockroach and mouse exposure and associations with asthma in families and elderly individuals living in New York City public housing.
• Distinguishing product and practice regulation in personalized medicine.
• Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase.
• Effects of platelet-activating factor and platelet-activating factor: acetylhydrolase on in vitro post-thaw boar sperm parameters.
• Embryo production from superovulated sheep inseminated with sex-sorted ram spermatozoa.
• Focal cortical atrophy in multiple sclerosis: relation to lesion load and disability.
• Homocysteine in health and disease.
• Is lower cognitive function in one spouse associated with depressive symptoms in the other spouse?
• Judith corker, 1967-1998.
• Maintenance of delay of gratification by four chimpanzees (Pan troglodytes): the effects of delayed reward visibility, experimenter presence, and extended delay intervals.
• Mechanism-based pharmacodynamic models of fluoroquinolone resistance in Staphylococcus aureus.
• Morphine analgesic tolerance in 129P3/J and 129S6/SvEv mice.
• Prodigious alcohol consumption by Australian rugby league footballers.
• Relative weight and mobility: a longitudinal study in a biracial population of older adults.
• STAT5 acts as a repressor to regulate early embryonic erythropoiesis.
• Serum estradiol-17beta concentrations during spontaneous silent estrus and after prostaglandin treatment in the mare.
• Smad1 expands the hemangioblast population within a limited developmental window.
• Strategies for treatment-resistant depression.
• Strategies for treatment-resistant depression.
• The acute effects of gabapentin in combination with alcohol in heavy drinkers.
• The effects of xylazine and alpha-adrenoreceptor antagonists on bovine uterine contractility in vitro.
• The evaluation of extracorporeal shock wave therapy on collagenase induced superficial digital flexor tendonitis.
• To the editor.
• Treatment approaches for interstitial cystitis: multimodality therapy.