Dual roles of 90-kDa heat shock protein in the function of the mineralocorticoid receptor.

Dual roles of 90-kDa heat shock protein in the function of the mineralocorticoid receptor. Research Abstract Details 

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Dual roles of 90-kDa heat shock protein in the function of the mineralocorticoid receptor. Abstract Text:

T Nemoto,Y Ohara-Nemoto,N Sato,M Ota,

Association of the 90-kDa heat shock protein (HSP90) is required for the high-affinity ligand-binding of the glucocorticoid receptor (GR), but not for that of the androgen receptor [Ohara-Nemoto, Y., Nemoto, T., & Ota, M. (1991) J. Biochem. 109, 113-119]. In the present study, we investigated the ligand- and HSP90-binding characteristics of the mineralocorticoid receptor (MR), which shares to some degree the ligand-binding specificity of the GR. A truncated human MR (designated MR351) starting from Gly-351 was translated in vitro with rabbit reticulocyte lysate. Scatchard analysis revealed the presence of a single class of high-affinity binding sites for [3H]aldosterone (Kd = 0.35 +/- 0.2 nM), comparable to those of the native receptor in target tissues. Glycerol gradient centrifugation and immunoadsorption analyses showed that MR351 associated with rabbit HSP90. Exposure to 0.4 M NaCl induced the dissociation of HSP90 from MR351 and simultaneously enhanced binding of MR351 to DNA-cellulose. Moreover, when measured at 10 nM, HSP90-free MR351 showed only 9% of the [3H]aldosterone-binding found in the presence of HSP90. On the other hand, when MR351 was expressed in Escherichia coli as a protein tagged with a histidine hexamer at the N-terminus (designated H6MR351), specific binding of [3H]aldosterone was detected. The binding affinity (Kd = 338 +/- 45 nM) was, however, 1,000-fold lower than that of MR351 translated in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)

Dual roles of 90-kDa heat shock protein in the function of the mineralocorticoid receptor. Publishing Authors By Initials

T Nemoto,Y Ohara-Nemoto,N Sato,M Ota,

For similar cells: blood cells: erythrocytes: reticulocytes research abstracts see: cells: blood cells: erythrocytes: reticulocytes research

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Dual roles of 90-kDa heat shock protein in the function of the mineralocorticoid receptor. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 113

Page Numbers: 769-75

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1993

Dual roles of 90-kDa heat shock protein in the function of the mineralocorticoid receptor. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Dual roles of 90-kDa heat shock protein in the function of the mineralocorticoid receptor. Keywords Mesh Terms:

KEYWORDS: Reticulocytes

MESH TERMS: metabolism

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Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Dual roles of 90-kDa heat shock protein in the function of the mineralocorticoid receptor.

AFFILIATION: Department of Biochemistry, Iwate Medical University School of Dentistry.

Country: JAPAN

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MEDLINETA: J Biochem

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