Dual compartmental localization and function of mammalian NADP+-specific isocitrate dehydrogenase in yeast.

Dual compartmental localization and function of mammalian NADP+-specific isocitrate dehydrogenase in yeast. Research Abstract Details 

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Dual compartmental localization and function of mammalian NADP+-specific isocitrate dehydrogenase in yeast. Abstract Text:

Qian Lu,Karyl I Minard,Lee McAlister-Henn,

Isozymes of NADP+-specific isocitrate dehydrogenase (IDP) provide NADPH in cytosolic, mitochondrial, and peroxisomal compartments of eukaryotic cells. Analyses of purified IDP isozymes from yeast and from mouse suggest a general correspondence of pH optima for catalysis and pI values with pH values reported for resident cellular compartments. However, mouse IDP2, which partitions between cytosolic and peroxisomal compartments in mammalian cells, exhibits a broad pH optimum and an intermediate pI value. Mouse IDP2 was found to similarly colocalize in both cellular compartments when expressed in yeast at levels equivalent to those of endogenous yeast isozymes. The mouse enzyme can compensate for loss of yeast cytosolic IDP2 and of peroxisomal IDP3. Removal of the peroxisomal targeting signal of the mouse enzyme precludes both localization in peroxisomes and compensation for loss of yeast IDP3.

Dual compartmental localization and function of mammalian NADP+-specific isocitrate dehydrogenase in yeast. Publishing Authors By Initials

Q Lu,KI Minard,L McAlister-Henn,

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Dual compartmental localization and function of mammalian NADP+-specific isocitrate dehydrogenase in yeast. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Archives of biochemistry and biophysics

VOLUME: 472

Page Numbers: 17-25

Journal Abbreviation: Arch. Biochem. Biophys.

ISSN: 1096-0384

DAY: 6

MONTH: 02

YEAR: 2008

Dual compartmental localization and function of mammalian NADP+-specific isocitrate dehydrogenase in yeast. Information

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LANGUAGE: eng

NlmUniqueID: 372430

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Grant and Affiliation Information for Dual compartmental localization and function of mammalian NADP+-specific isocitrate dehydrogenase in yeast.

AFFILIATION: Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229, USA.

Country: United States

AGENCY: United States NIA

GRANT: AG17477

ACRONYM: AG

MEDLINETA: Arch Biochem Biophys

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