dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae.

dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae. Research Abstract Details 

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dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae. Abstract Text:

Xuewen Pan,Daniel S Yuan,Siew-Loon Ooi,Xiaoling Wang,Sharon Sookhai-Mahadeo,Pamela Meluh,Jef D Boeke,

Analysis of genetic interactions has been extensively exploited to study gene functions and to dissect pathway structures. One such genetic interaction is synthetic lethality, in which the combination of two non-lethal mutations leads to loss of organism viability. We have developed a dSLAM (heterozygote diploid-based synthetic lethality analysis with microarrays) technology that effectively studies synthetic lethality interactions on a genome-wide scale in the budding yeast Saccharomyces cerevisiae. Typically, a query mutation is introduced en masse into a population of approximately 6000 haploid-convertible heterozygote diploid Yeast Knockout (YKO) mutants via integrative transformation. Haploid pools of single and double mutants are freshly generated from the resultant heterozygote diploid double mutant pool after meiosis and haploid selection and studied for potential growth defects of each double mutant combination by microarray analysis of the "molecular barcodes" representing each YKO. This technology has been effectively adapted to study other types of genome-wide genetic interactions including gene-compound synthetic lethality, secondary mutation suppression, dosage-dependent synthetic lethality and suppression.

dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae. Publishing Authors By Initials

X Pan,DS Yuan,SL Ooi,X Wang,S Sookhai-Mahadeo,P Meluh,JD Boeke,

For similar fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research abstracts see: fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research

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dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Methods (San Diego, Calif.)

VOLUME: 41

Page Numbers: 206-21

Journal Abbreviation: Methods

ISSN: 1046-2023

DAY: 3

MONTH: Feb

YEAR: 2007

dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae. Information

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LANGUAGE: eng

NlmUniqueID: 9426302

dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae

MESH TERMS: genetics

Chemical & Substance for Abstract: dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae. Information

Substance Name: Oligonucleotides

Registry Number: 0

Grant and Affiliation Information for dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae.

AFFILIATION: Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA.

Country: United States

AGENCY: United States NCRR

GRANT: U54 RR020839-02

ACRONYM: RR

MEDLINETA: Methods

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