Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells.

Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells. Research Abstract Details 

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Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells. Abstract Text:

Kaori Shimanouchi,Kei-Ichi Takata,Masamitsu Yamaguchi,Shizuka Murakami,Gen Ishikawa,Ryo Takeuchi,Yoshihiro Kanai,Tatsushi Ruike,Ryoichi Nakamura,Yoko Abe,Kengo Sakaguchi,

The damaged DNA-binding protein (DDB) complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2) subunits and is believed to have a role in nucleotide excision repair (NER). We used the GAL4-UAS targeted expression system to knock down DDB1 in wing imaginal discs of Drosophila. The knock-down was achieved in transgenic flies using over-expression of inverted repeat RNA of the D-DDB1 gene [UAS-D-DDB1(650)-dsRNA]. As a consequence of RNA interference (RNAi), the fly had a shrunken wing phenotype. The wing spot test showed induced genome instability in transgenic flies with RNAi knock-down of D-DDB1 in wing imaginal discs. When Drosophila larvae with RNAi knock-down of D-DDB1 in wing imaginal discs were treated with the chemical mutagen methyl methanesulfonate (MMS), the frequency of flies with a severely shrunken wing phenotype increased compared to non-treated transgenic flies. These results suggested that DDB1 plays a role in the response to DNA damaged with MMS and in genome stability in Drosophila somatic cells.

Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells. Publishing Authors By Initials

K Shimanouchi,K Takata,M Yamaguchi,S Murakami,G Ishikawa,R Takeuchi,Y Kanai,T Ruike,R Nakamura,Y Abe,K Sakaguchi,

For similar animal structures: forelimb: wing research abstracts see: animal structures: forelimb: wing research

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Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 139

Page Numbers: 51-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 2006

Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells. Keywords Mesh Terms:

KEYWORDS: Wing

MESH TERMS: growth & development

Chemical & Substance for Abstract: Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells. Information

Substance Name: DNA-Binding Proteins

Registry Number: 0

Grant and Affiliation Information for Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells.

AFFILIATION: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510.

Country: Japan

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MEDLINETA: J Biochem

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