Downregulation of renal TonEBP in hypokalemic rats.

Downregulation of renal TonEBP in hypokalemic rats. Research Abstract Details 

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Downregulation of renal TonEBP in hypokalemic rats. Abstract Text:

Un Sil Jeon,Ki-Hwan Han,Soo-Hyun Park,Sang Do Lee,Mee Rie Sheen,Ju-Young Jung,Wan Young Kim,Jeff M Sands,Jin Kim,H Moo Kwon,

Hypokalemia causes a significant decrease in the tonicity of the renal medullary interstitium in association with reduced expression of sodium transporters in the distal tubule. We asked whether hypokalemia caused downregulation of the tonicity-responsive enhancer binding protein (TonEBP) transcriptional activator in the renal medulla due to the reduced tonicity. We found that the abundance of TonEBP decreased significantly in the outer and inner medullas of hypokalemic rats. Underlying mechanisms appeared different in the two regions because the abundance of TonEBP mRNA was lower in the outer medulla but unchanged in the inner medulla. Immunohistochemical examination of TonEBP revealed cell type-specific differences. TonEBP expression decreased dramatically in the outer and inner medullary collecting ducts, thick ascending limbs, and interstitial cells. In the descending and ascending thin limbs, TonEBP abundance decreased modestly. In the outer medulla, TonEBP shifted to the cytoplasm in the descending thin limbs. As expected, transcription of aldose reductase, a target of TonEBP, was decreased since the abundance of mRNA and protein was reduced. Downregulation of TonEBP appeared to have also contributed to reduced expression of aquaporin-2 and UT-A urea transporters in the renal medulla. In cultured cells, expression and activity of TonEBP were not affected by reduced potassium concentrations in the medium. These data support the view that medullary tonicity regulates expression and nuclear distribution of TonEBP in the renal medulla in cell type-specific manners.

Downregulation of renal TonEBP in hypokalemic rats. Publishing Authors By Initials

US Jeon,KH Han,SH Park,SD Lee,MR Sheen,JY Jung,WY Kim,JM Sands,J Kim,HM Kwon,

For similar proteins: carrier proteins: membrane transport proteins: ion pumps: symporters: sodium-potassium-chloride symporters research abstracts see: proteins: carrier proteins: membrane transport proteins: ion pumps: symporters: sodium-potassium-chloride symporters research

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MEDLINE DATE:

Downregulation of renal TonEBP in hypokalemic rats. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Renal physiology

VOLUME: 293

Page Numbers: F408-15

Journal Abbreviation: Am. J. Physiol. Renal Physiol.

ISSN: 0363-6127

DAY: 4

MONTH: 04

YEAR: 2007

Downregulation of renal TonEBP in hypokalemic rats. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901990

Downregulation of renal TonEBP in hypokalemic rats. Keywords Mesh Terms:

KEYWORDS: Sodium-Potassium-Chloride Symporters

MESH TERMS: metabolism

Chemical & Substance for Abstract: Downregulation of renal TonEBP in hypokalemic rats. Information

Substance Name: sodium-potassium chloride cotransporter

Registry Number: 0

Grant and Affiliation Information for Downregulation of renal TonEBP in hypokalemic rats.

AFFILIATION: Dept. of Medicine, University of Maryland, Baltimore, MD 21201, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK-61677

ACRONYM: DK

MEDLINETA: Am J Physiol Renal Physiol

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